Do you know the classification of glutamate dehydrogenase (GDH)?
Most organisms contain glutamate dehydrogenase (GDH) (E.C.220.127.116.11–18.104.22.168). In eukaryotes, this enzyme is a mitochondrial enzyme, which is ubiquitous in animals, plants and microorganisms. It belongs to the superfamily of amino acid dehydrogenases and plays a key role in the metabolism of nitrogen and carbon in organisms. Do you know the classification of glutamate dehydrogenase? What are their characteristics? If we want to understand it more deeply, we must continue to dig it so that we can use it better and create more miracles for our lives.
1. Classification according to oligomer status
Existing research reports have isolated glutamate dehydrogenase from many types of organisms and sequenced them. According to the state of oligomers, glutamate dehydrogenase can be divided into two types: NADP(H) specific glutamate dehydrogenase of bacteria and fungi and NAD(H)/NADP(H) of vertebrates Bispecific glutamate dehydrogenase. These GDHs all function in the form of homohexamers, and the molecular weight of each subunit is about 48kDa (bacteria) or 50kDa (vertebrate); and NAD(H) specific glutamate dehydrogenase has existing subunits Homohexameric proteins with a molecular weight of about 48kDa (such as Peptostreptococcus and Clostridium symbiotic) also have homotetrameric proteins with a subunit molecular weight of about 115kDa (such as Saccharomyces cerevisiae and Alternaria crassa).
2. According to the specific classification of coenzymes
Glutamate dehydrogenase requires NAD+/NADP+ as a coenzyme when catalyzing the oxidative deamination reaction of L-glutamate. According to the specificity of the coenzyme, glutamate dehydrogenase can be divided into three categories: NAD(H) specificity Glutamate dehydrogenase, NADP(H) specific glutamate dehydrogenase and NAD(H)/NADP(H) dual specific glutamate dehydrogenase. Different specific glutamate dehydrogenases have different functional biases. NAD(H) specific glutamate dehydrogenase is mainly involved in the catabolism of glutamate, and NADP(H) specific glutamate dehydrogenase Hydrogenase usually plays a role in ammonia synthesis and metabolism. The NAD(H)/NADP(H) bispecific glutamate dehydrogenase will be allosterically regulated by some small molecules (such as ATP and GTP) when it plays a catalytic role. ATP can accelerate the rate of catalytic reaction through the release of auxiliary products, while GTP can prevent the release of products and inhibit the catalytic reaction.
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