Ketotifen fumarate is a second-generation anti-allergic drug, mainly used to treat bronchial asthma, allergic rhinitis, eczema and other diseases. At present, the main methods for determining ketotifen fumarate include spectrophotometry, high performance liquid chromatography, membrane selective electrode method and capillary electrophoresis.

The chemiluminescence analysis method is widely used in the direct determination of drugs and biochemical samples because of its high sensitivity, wide linear range, and simple instrument operation. Studies on the determination of ketotifen fumarate by chemiluminescence and electrochemiluminescence analysis have also been reported. However, the study on the determination of ketotifen fumarate based on potassium permanganate chemiluminescence system has not been reported yet. Experiments have found that in alkaline medium, potassium permanganate can oxidize Lumino1 to produce a luminescent signal, and ketotifen fumarate has a strong sensitizing effect on the luminescent signal.

For this reason, our engineer Xiong from the Desheng Technology Department conducted an experiment and analyzed and reached the following conclusions:

1. The flow injection chemiluminescence signal of the flow injection chemiluminescence signal system is shown in the figure.

It can be seen from the figure that in an alkaline medium, potassium permanganate can oxidize luminol to produce chemiluminescence. When ketotifen fumarate is added to the reaction system, the chemiluminescence intensity is significantly enhanced.

2. Chemiluminescence spectrum

Turn off the fluorescence spectrophotometer light source, draw the chemiluminescence spectra of luminol-potassium permanganate-ketotifen fumarate solution and luminol-potassium permanganate solution

It can be seen from the figure that the maximum emission wavelength of the two luminescence systems is about 425 nm, which is consistent with the maximum emission wavelength of the luminol luminophore, indicating that the luminophores of the two chemiluminescence systems are excited state 3-aminophthalene Formate. The luminescence intensity of the luminol-potassium permanganate-ketotifen fumarate system is significantly higher than that of the luminol-potassium permanganate system, indicating that the addition of ketotifen fumarate enhances the sensitivity of luminol-permanganate The luminous intensity of the potassium acid system.

3. Choice of road and flow

The experiment found that different mixing methods of the solution have a great influence on the luminescence signal. The multiple flow paths of the selected system were compared, and the results showed that the flow path shown in Figure 1 had a stable and large luminous signal, so this flow path was selected for analysis. The influence of different flow rates on the luminescence signal of the system was investigated. The experiment shows that with the increase of the flow rate, it first increases and then decreases, and reaches the maximum value when the flow rate reaches 1.9 mL·min. The test selects the flow rate of each pipeline to be 1.9 mL·min.

4. Selection of the concentration of sodium hydroxide solution

In 0.02～0.3 mol·L-4. Within the scope, the influence of the concentration of sodium hydroxide solution on the luminescence intensity of the reaction system was investigated. The results show that when the concentration of sodium hydroxide solution is 0.05～0.15Mol·L-1, the maximum and basically unchanged, the test chooses the concentration of sodium hydroxide solution to be 0.1 Mol·L-4.

5. The choice of oxidant and its concentration

The experiment investigated the influence of different oxidants such as potassium permanganate, potassium ferricyanide, hydrogen peroxide, potassium periodate on the chemiluminescence signal of the system. The results show that when potassium permanganate is used as the oxidant, the chemiluminescence sensitivity signal value is large and stable. Its concentration is 5.0×10-4mol·L-1. Therefore, the concentration of potassium permanganate solution is 5.0×10-4mol·L-1.

6. The choice of the concentration of luminol solution

Luminol is the main luminescent substance, and its concentration has a greater impact on the luminous intensity, detection sensitivity, and linear range. The experiment investigated the influence of luminol solution concentration in the range of 2.0×10-4～1.0×10-4mol·L_1 on the luminescence signal of the system. The results showed that as the concentration of luminol solution increased, chemiluminescence The increasing intensity indicates that the concentration of the luminol solution should not be too large; if the concentration of the luminol solution is too small, the luminescence signal value is small and the stability is poor. In consideration of stability and sensitivity, the concentration of luminol solution was selected as 3.0×10-4mol·L-1.

7. Selection of sensitizer

The test investigated the surfactants polyvinyl alcohol, sodium dodecyl sulfonate, cetylpyridinium chloride, emulsifier, sodium dodecylbenzene sulfonate, cetyltrimethylammonium bromide, The influence of sodium lauryl sulfate, icyclodextrin, nano silver, nano gold, nano platinum, etc. as sensitizers on the luminescence signal of the system. The results show that the addition of the above sensitizer has no obvious sensitizing effect on the luminous intensity of the system.

Desheng is a professional R&D and manufacturer of chemiluminescence reagents. The raw materials of chemiluminescence reagents include luminol, isoluminol and 6 commonly used acridinium esters (not detailed here, please contact customer service on the official website if you need it) Product purity ≥98%, high chemiluminescence efficiency. In addition to chemiluminescence reagents, it can also provide a variety of in vitro diagnostic reagents. Friends in need are welcome to inquire and order.