12 uses of HEPES Buffer you didn't know
Release time:
2020-11-15
Hepes is a zwitterionic biological buffer, commonly used in biological and biochemical research. It is one of the earliest Good buffers described by Norman E. Good in 1966. Although it is a very popular buffer in laboratories around the world, what is Hepes actually suitable for? The following can better understand the characteristics of Hepes and its applicability to different biotechnology applications (cell culture, chromatography, etc.). To
Basic Information:
CAS Number: 7365-45-9
Molecular weight: 238.3
Molecular formula: C 8 H 18 N 2 O 4 S
Useful pH range: 6.8-8.2
pKa (25°C): 7.45-7.65
Maximum wavelength (λmax)λ: 260 nm Amax: 0.06; λ: 280 nm Amax: 0.04
What can Hepes do?
ØResearch on most metal ions
ØIt can be a good substitute for Tris and phosphate in metal ion research
ØUsed for environmental, analytical and biological research
ØAs the binding buffer and eluent in cation exchange chromatography
ØAs a grinding buffer in plant research
ØAs a running buffer in electrophoresis
ØAs a buffer for electroporation
ØBuffer mammalian cell culture
ØAs a buffer for in vitro fertilization and embryo culture
ØFor Bradford or bicinchoninic acid (BCA) analysis
ØFor research on cartilaginous amphibians, because it is non-toxic to this algae
ØIt has been introduced into the extraction buffer to prevent damage to the proteins in red blood cells
What should you consider before choosing Hepes for research?
uIt affects the membrane potential of neuronal cells
uIt will interfere with the determination of Lowry nucleic acid protein
uIt is not suitable for redox research
uIt is toxic to small crustaceans (water fleas), such as Daphnia magna and Daphnia pulex
uIt will interfere with the phenol oxidation of peroxidase
uIt will affect the self-oxidation rate of iron
uIt is not recommended to use Folin reagent for protein determination
Useful tips about Hepes:
²Easily soluble in water
²It has negligible metal ion bond
²It can inactivate the popular RNase inhibitor DEPC
In the specific use of hepes, cosmetics are used as pH adjustment, which is conducive to the formation and stability of gold nanostars. It is also present in the prevailing virus preservation solutions today, which can be used for a wide range of purposes.
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