In the precise chain of clinical testing, every step from blood sample collection to machine testing directly affects the accuracy of the results. Among them, the sample processing steps after using coagulation tubes for blood collection may seem simple, but they contain scientific principles that cannot be ignored. The standardized use of coagulants is not only a requirement of standard operating procedures, but also a key factor in ensuring serum quality, avoiding pre analysis errors, and ensuring the normal operation of detection instruments.

coagulants

1、 Common problems with improper use of coagulants

Improper use of coagulants can lead to various sample quality issues. Insufficient mixing of coagulants is the primary issue, which can lead to incomplete conversion between blood cells and fibrinogen, manifested as: 1. Blood cells and fibrin filaments hanging on the wall 2. Mild hemolysis, interfering with detection results 3. Collagen precipitation 4. No serum separation after centrifugation

The deeper risk lies in the insufficient action time of the coagulant. When the clotting time is less than 5 minutes, centrifugation can cause secondary precipitation of fibrin filaments. These fibrin fibers float in the serum and easily block the precision probes of the fully automated biochemical analyzer. This not only affects the detection of individual samples, but may also cause instrument malfunctions and interrupt batch sample testing.

2、 Analysis of standard operating procedures for coagulants

The standardized use of coagulants requires following scientifically rigorous operating procedures: after blood collection during the coagulant mixing stage, 5-8 gentle reversals should be immediately performed to mix well. The key to this step is "gentle and sufficient": vigorous shaking can cause hemolysis, and insufficient mixing can result in uneven distribution of coagulants. The purpose is to ensure uniform dispersion of the coagulant and activate the coagulation cascade reaction. After mixing during the coagulant action stage, the blood collection tube should be left vertically at room temperature of 20-25 ℃ for 5-8 minutes. This is the critical period of coagulation reaction. The risk of insufficient action time of coagulants: If the settling time is less than 5 minutes, the polymerization of fibrin will be unstable, and centrifugal force can damage the clot structure, leading to the re precipitation of fibrin filaments.

3、 Quality control management of coagulant use

Establishing systematic quality management is the key to ensuring sample quality:

Standardization of coagulant operation: Incorporate the use specifications of coagulants into standard operating procedures, and strengthen the understanding of the scientific principles of each step by training operators, shifting from "doing as required" to "understanding why to do it".

Monitoring of coagulant effect: Establish a sample quality evaluation mechanism, using the presence of fibrin fibers, hemolysis, and intact clots in serum as sample acceptance criteria, to achieve standardized operation from feedback of results.

Selection of coagulant products: Choose high-quality coagulant products with stable performance and high efficiency, which can maintain stable performance over a wider range of temperatures and blood viscosity, providing a more reliable quality control basis for clinical operations.

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Accurate detection begins with standardized operations and originates from high-quality raw materials. Hubei Xindesheng Material Technology Co., Ltd., as a professional supplier of in vitro diagnostic raw materials, provides high-quality coagulant series products with stable and reliable performance and professional technical support, helping laboratories obtain high-quality serum samples. Welcome medical institutions and industry colleagues to visit and exchange ideas, explore cooperation, and jointly improve inspection quality.