How to confirm if blood is completely clotted
The principle of blood coagulation: The process in which blood changes from a flowing liquid state to a jelly-like clot that cannot flow is known as blood coagulation. This is a series of limited protein hydrolysis processes involving clotting factors.
The principle of blood coagulation: blood coagulation is abbreviated as coagulation, which is the process of blood changing from a flowing state to a gel state. It is an important part of the hemostatic function. The coagulation process is a process in which a series of coagulation factors are activated by successive enzymolysis, and finally thrombin is formed to form a fibrin clot. So far, there are 14 factors involved in blood coagulation. Among them, 12 are numbered with Roman numerals (from Ⅰ to Ⅷ, where factor Ⅵ does not exist).
blood collection tube additives:blood coagulation
The key process of blood coagulation is the conversion of fibrinogen in plasma to insoluble fibrin. The polymer fibrin is interwoven into a network, which entrains many blood cells to form a blood clot. 1 to 2 hours after the blood clotting process, the blood clot shrinks and precipitates under the action of platelets and is a pale yellow liquid called serum. Compared with plasma, serum lacks fibrinogen and a small amount of other plasma proteins involved in blood coagulation, and adds a small amount of substances released by platelets during blood coagulation.
The significance of the coagulation tube: After the blood of the human body is drawn into the coagulation tube, a large amount of substances that can activate blood coagulation are added to the coagulation tube, which shortens the blood coagulation process that would otherwise take 1-2 hours to 15- 30 minutes, which greatly saves the hospital's testing time and allows doctors to quickly obtain the test results, so the coagulation tube is widely used in hospitals.
How to judge that the blood is completely coagulated:
Initial stage: Slightly shake the coagulation tube, the blood can flow freely in the coagulation tube.
Slight coagulation stage: Slightly shake the coagulation tube, the blood does not flow in the tube.
Intermediate coagulation stage: Slightly shake the coagulation tube, the blood does not flow in the tube and there is serum precipitation.
Deep coagulation state: a large amount of serum is precipitated, and the coagulation tube is slightly shaken, and the blood clot can slide back and forth in the tube because of the complete contraction.
When oxalate, sodium citrate, and EDTA are added to the blood, the anticoagulant effect is due to the removal of calcium ions; the reason why the blood does not coagulate in the blood vessel is because in the normal blood vessel, the exposure factor is not Activation and the presence of anticoagulant factors (antithrombin, heparin, etc.) in the plasma.
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