Luminol reagent, due to its chemiluminescence properties, has been widely used in criminal investigation, biomedical research, environmental monitoring and other fields. Whether it is detecting trace bloodstains at crime scenes or labeling biomolecules in laboratories, luminol plays an irreplaceable role. However, luminol reagents are extremely sensitive to light, and their storage and use have strict requirements. Proper light avoidance storage and standardized use are key to ensuring stable performance and accurate detection results of luminol reagents.

Luminol powder

Properties of Luminol Reagent

Luminol, under normal circumstances, is a yellow powder, and its chemical structure determines its unique chemiluminescence properties. Under alkaline conditions, luminol can be oxidized by certain oxidants (such as hydrogen peroxide) to form unstable intermediates. When these intermediates decompose, photons are released, resulting in chemiluminescence. However, this chemical structure also makes luminol sensitive to light, which accelerates its oxidation reaction and causes the reagent to decompose and deteriorate.

Light shielded storage method for luminol reagent

1. Choose appropriate packaging container: Luminol reagents should be stored in opaque containers such as brown glass bottles or aluminum foil bags. Brown glass bottles can block ultraviolet and visible light, reducing the impact of light on reagents; Aluminum foil bags have good light and moisture resistance, which can further protect reagents. During use, try to avoid transferring the luminol reagent to transparent containers as much as possible. If it needs to be divided, choose small brown bottles and ensure good sealing.

2. Control storage environment: In addition to avoiding light, the temperature and humidity of the storage environment for luminol reagents also need to be strictly controlled. Generally speaking, luminol reagents should be stored in a cool and dry place. Excessive temperature can accelerate the decomposition of luminol, while excessively low temperature may cause the reagent to crystallize, affecting its effectiveness. In addition, environments with high humidity can cause the luminol reagent to absorb moisture, thereby affecting its chemical properties and luminescence performance.

3. Reduce exposure time of reagents: When using luminol reagents, the exposure time to light should be minimized as much as possible. During the operation, quickly open the container, take the required amount of reagent quickly, and immediately seal the container. If it is necessary to prepare luminol solution, it should be done in a dark room or in a dimly lit environment, and it should be prepared on the spot to avoid long-term storage of the solution.

Taboos for the use of luminol reagent

1. Avoid mixing with strong oxidants: Although luminol requires the action of oxidants to emit light, not all oxidants are suitable for luminol reactions. Strong oxidants such as potassium permanganate and potassium dichromate may react violently with luminol, leading to uncontrolled luminescence and even causing danger.

2. Pay attention to the pH value of the reaction system: The chemiluminescence reaction of luminol requires a strict pH value for the reaction system. Generally speaking, in alkaline environments, the luminescence efficiency of luminol is high. If the pH value is too high or too low, it will affect the oxidation reaction of luminol, resulting in a decrease in luminescence intensity or even no luminescence. Before using the luminol reagent, it is necessary to adjust the pH value of the reaction system to an appropriate range with a buffer solution and maintain the stability of the pH value during the reaction process.

3. Preventing interference from impurities: Luminol reagent has high detection sensitivity, making it highly susceptible to interference from impurities. During use, it is necessary to ensure that the experimental equipment is clean and avoid introducing impurities such as metal ions and organic matter. Metal ions such as iron ions and copper ions may catalyze the oxidation reaction of luminol, leading to enhanced background luminescence and affecting the accuracy of detection results. When using luminol reagent to detect bloodstains at a criminal investigation site, attention should also be paid to the presence of other substances in the environment that may interfere with the detection, such as certain cleaning agents, chemicals, etc.

Product packaging

Hubei Xindesheng Company has demonstrated strong capabilities in the research and production of luminol reagents. The company relies on years of industry experience and professional technology to produce high-quality reagents such as luminol and isoluminol. New Desheng strictly controls every link in the production process of luminol reagents, from raw material procurement to finished product packaging, all following standard quality requirements. The luminol reagent produced by it has high purity, low impurity content, stable luminescence performance, and has won a good reputation in the market. If you have any purchasing needs for luminol, please feel free to click on the website for consultation at any time! ‌