The most ideal biological buffer in history-blood
From phosphate buffers to Tris buffers, to TEA, TEB, HEPES, etc., biological buffers are becoming more and more useful, and more and more applications, such as enzyme-catalyzed reactions, antibody incubation, cell culture, virus preservation solution , Nucleic acid PCR, etc. basically run through the entire biochemical field, but the most ideal biological buffer is given to us by nature-blood.
There are three main types of biological buffer systems in human blood: bicarbonate and carbonate buffer systems, biphosphate and dihydrogen phosphate buffer systems, and plasma protein (plasma protein, hemoglobin, oxygen, and hemoglobin) buffer systems. The bicarbonate buffer system is the most important.
Blood and biological buffer
Blood as a buffer has good compatibility
We know that for biochemical testing, we need to use corresponding buffers according to different experiments, such as Tris for DNA electrophoresis buffer, CAPS for Western blotting, HEPES for
virus preservation solution non-inactivation, etc., and for different enzymes involved in biochemical testing The buffer should also be selected according to the optimal pH of the corresponding enzyme. But for blood, it can be compatible with various biochemical reactions of various physiological activities in the body at the same time, and can accurately control the catalytic performance of various enzymes. This compatibility is beyond the reach of our biological buffers.
Blood is fully functional
In general, the buffer solution needs to be improved in some aspects. For example, Tris is used in Southern blotting experiments to add EDTA salt to increase the conductivity. HEPES is used in virus preservation solution. It also needs to add glucose, amino acids and other nutrients. Some need to adjust the osmotic pressure and add preservatives. The blood has a very comprehensive function. It can transport oxygen to provide energy, provide nutrients, regulate temperature and other functions. It even has immune functions to resist germs. It can be described as self-preservation.
As can be clearly seen from the above points, the performance of blood in buffers far exceeds that of various buffers that we artificially produce. As a biological buffer manufacturer, desheng hope that one day can produce a buffer comparable to blood. Just like this slim universe, the miracle of life appears after all.
HEPES, as a zwitterionic buffer, increases the osmotic pressure of the cell culture system by increasing the concentration of solution ions, maintaining normal cell morphology and function, and improving cell survival rate. Widely used in cell culture, especially under specific conditions such as tumor cell culture, it is crucial to maintain cell growth and function.