How to describe in vitro diagnostic reagents
After the baptism of the epidemic situation, the attention of the in vitro diagnostic industry continues to rise, and the development potential is huge. Many newcomers have also moved to this industry. However, whether it is a manufacturing company or a trading company, business or procurement, they are very new to in vitro diagnostic reagents. It is very helpful to promote a wide variety of in vitro diagnostic reagents.
For the classification of in vitro diagnostic reagents, there are many different classifications in different fields. Understanding the product from different angles determines the degree of understanding, acceptance and memory of the reader. We will classify the biochemical detection from simple to complex.
1. All kinds of water
Many people think that it is just water, which can be seen everywhere. What kind of reagent is this? Experienced people know that for different experiments, the first thing to confirm is what kind of water needs to be removed, such as distilled water, secondary water, deionized water, ultra-pure water, etc. Although ordinary tap water has been purified, there are still many impurity ions for biochemical detection. Distilled water is water condensed by water vapor after boiling, and secondary water is cyclic distillation twice, which is a process to remove impurity ions.
Various in vitro diagnostic reagents
2. Organic solvents such as ethanol, ether and acetone
The most basic function of water in biochemical detection experiments is to serve as a solvent, but some substances are not soluble in water, or for some special needs, organic solvents such as ethanol, ether, and acetone are used. For example, accelerators and oily silicifying agents usually use ethanol or ether as a solvent; carbomer is used as a solvent in skin care gels, and alcohol is used as a solvent in no-clean disinfectants. Regardless of whether it is an aqueous solvent or other solvents, bio-buffering (Tris, Bicine, etc.) usually needs to be added to maintain a suitable reaction environment.
Third, color reagents, luminous reagents and other indicating reagents
The biochemical detection value can be detected because a biochemical reaction has occurred, but if this reaction cannot be directly observed, we cannot judge the detection result. Therefore, it is necessary to use suitable indicators, there are many types, such as pH indicator methyl orange, Feilin reagent, biuret reagent and Trinder's color reagent TOOS, MAOS, etc.; chemiluminescent reagents such as Luminol , Acridinium ester, AMPPD, etc. In addition to color development and chemiluminescence, there are turbidimetric methods that generate precipitates or colloids and detection of related reagents based on changes in current.
4. Reagents involved in the reaction
There are many reagents involved in the reaction in biochemical detection, most of which are organic reagents, usually sugars, lipids, proteins, amino acids, antigens and antibodies. Since biochemical detection requires high sensitivity and fast reaction speed, the most commonly used reactions are those catalyzed by enzymes or reactions that specifically bind antigens and antibodies. Enzymes and antibodies are special proteins, and most reactions in the body are catalyzed by enzymes.
Through the overall classification of different in vitro diagnostic reagents, we can understand that for a biochemical test project, the approximate role of a certain reagent, the price ratio, etc., also facilitate the understanding of a substance used in industry, food, biochemical testing and The difference between medicine and whether it is the required reagent. If you want to further understand the various in vitro diagnostic reagents, you can also directly contact Desheng website.
The Lumino coupling indirect determination method is used to detect trace substances in biological samples, which has the advantages of high sensitivity and simple operation. But accuracy is affected by the sample matrix, experimental conditions, and interfering substances. When using, attention should be paid to sample representativeness, control of experimental conditions, and elimination of interference, while comparing with other detection methods to evaluate accuracy.