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Biological buffers
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Trie (hydroxymethyl) aminomethane acetate TAE
CAS6850-28-8
Chinese Name: trimethylolminomethane acetate
English Name: tris (hydroxymethyl) aminomethane acetate salt
CAS No.: 6850-28-8
Molecular formula: C4H11NO3•C2H4O2
Molecular weight: 181.19
Storage conditions: Store at RT
Application: TAE electrophoresis buffer is the most commonly used buffer for DNA agarose gel electrophoresis, and is also used in non denatured RNA agarose gel electrophoresis. Double stranded DNA tends to move faster in TAE than in other buffers, but it will also be unable to swim due to the depletion of buffer ions during long-term electrophoresis. Buffer circulation or replacement during long-term electrophoresis can compensate for the low buffering capacity of the chemical book. Dilute the concentrated TAE buffer to obtain 1mmtae buffer, containing 40mMTris acetate and 1mMEDTA, with a pH of 8.3. 1mMTAE buffer can be used in agarose gel as well as electrophoretic buffer. In order to achieve the maximum resolution, it is recommended that the applied voltage be less than 5V/cm (unit distance between electrodes). TAE electrophoresis buffer is the most commonly used buffer for DNA agarose gel electrophoresis, and is also used in non denatured RNA agarose gel electrophoresis. Double stranded DNA tends to move faster in Tae than in other buffers, but it will also be unable to swim due to the depletion of buffer ions during long-term electrophoresis. The low buffering capacity of chemical book can be compensated by buffer circulation or buffer replacement during long-term electrophoresis. Dilute the concentrated TAE buffer to obtain 1mMTAE buffer, containing 40mMTris acetate and 1mMEDTA, with a pH of 8.3. 1mMTAE buffer can be used in agarose gel as well as electrophoretic buffer. In order to achieve the maximum resolution, it is recommended that the applied voltage be less than 5V/cm (unit distance between electrodes). In the detection of luciferase ATP with firefly, the product is the most sensitive buffer; glutamate binding detection.
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