Tricarboxymethylaminomethane acetate (TAE)

Alias: TRIS acetic acid, TRIS-acetic acid buffer
Molecular formula: C4H11NO3.C2H4O2
Molecular weight: 181.19
CAS: 6850-28-8
Purity: 99%
Melting point: 120-121°C
Boiling point: 219-220°C (9.7513mmHg)
Density: 1.09g/mLat20°C
Storage conditions: room temperature
Solubility: H2O: 0.5Mat20°C, clear, colorless
Form: solution
color: White     
Odor: Acetic acid
PH value: 6.0-7.0 (25℃, 0.5MinH2O)
PH range of acid-base indicator discoloration: 6-7at91g/lat25°C
Water solubility: 91g/lat20°C-completely soluble
Maximum wavelength (λmax)λ:260nmAmax:0.06λ:280nmAmax:0.05

use:
TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis, and it is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to move faster in TAE than in other buffers, but during long-term electrophoresis, the buffer ions are depleted and cannot migrate. Buffer circulation or buffer replacement during long-term electrophoresis can make up for the lower Chemicalbook buffer capacity. 2 Dilute the concentrated TAE buffer to obtain 1mMTAE buffer, containing 40mMTris acetate and 1mM EDTA, pH 8. 3. 1mMTAE buffer can be used in agarose gels, can also be used as an electrophoresis buffer. In order to achieve the maximum resolution, it is recommended that the applied voltage be lower than 5V/cm (the distance between the unit electrodes).