In the laboratory, serum separation gel is a common biochemical separation material used to separate serum from blood cells. However, there may be some problems during the use of separation adhesive, one of which is the common problem of probe blockage. Probe blockage can seriously affect the accuracy and reliability of experimental results. Therefore, it is very important to understand how to use separation gel correctly to avoid probe blockage.

The function and principle of serum separation gel

Serum separation gel is a special biomaterial with high specific gravity and viscosity. It can form a gel film in the blood, separating serum and blood cells. The principle of separation gel is to use physical and chemical properties to form different layers of serum and blood cells during the centrifugation process, which facilitates subsequent experimental operations.

The reason for probe blockage

The main reason for probe blockage is the precipitation of fibrin filaments in the serum. When the blood coagulation time is too long or too short, the specific gravity of serum separation gel is too large or too small, and the centrifugation speed is too fast or too slow, it may cause fibrin filaments to precipitate and attach to the probe, resulting in blockage. In addition, if the blood collection vessel is not clean, the quality of serum separation gel is poor, or there are problems during the packaging process, it may also cause probe blockage.

The correct method of using separation adhesive

It is crucial to use the separation gel correctly to prevent probe blockage. The following are the correct methods for using separation glue:

1. Control coagulation time: Excessive or insufficient coagulation time can lead to the precipitation of fibrin filaments. Therefore, it is necessary to control the coagulation time well and keep it within an appropriate range. Generally speaking, a coagulation time between 5-15 minutes is more appropriate.

2. Choosing the appropriate separation adhesive: It is very important to choose a separation adhesive with appropriate density and reliable quality. Different brands and models of separation adhesives have different densities, and it is necessary to choose the appropriate separation adhesive according to experimental requirements.

3. Centrifuge speed and time: Centrifuge speed too fast or too slow can lead to the precipitation of fibrin filaments. Generally speaking, a centrifugal speed between 2000-3000 revolutions per minute is more suitable. The centrifugation time should also be appropriately controlled, usually around 10-20 minutes.

4. Pay attention to the blood collection and packaging process: Unclean blood collection or problems during packaging may also cause probe blockage. Therefore, to ensure the cleanliness and sterility of blood collection vessels, the packaging process should be smooth and uniform.

5. Cleaning and maintaining probes: Regular cleaning and maintenance of probes can reduce the possibility of blockage. Use specialized probe cleaning agents for cleaning and regularly check if the probe is unobstructed.

6.Operating standards: Strictly follow the operating procedures for experimental operations to avoid improper operations that may cause fibrin filament precipitation or contamination.

The correct use of serum separation gel is the key to avoiding probe blockage. By controlling coagulation time, selecting appropriate serum separation gel, controlling centrifugation speed and time, paying attention to blood collection and packaging processes, cleaning and maintaining probes, and standardizing operations, the possibility of probe blockage can be effectively reduced, and the accuracy and reliability of experimental results can be improved.

As an advantageous supplier of blood vessel additives, Desheng is a high-tech industry that integrates self-developed, self-produced, and self sold products. We have rich experience in research and development technology in the preparation of separation gels, stable production performance, and mature processes. We provide high-quality serum separation gels for domestic and foreign customers. Please feel free to call us for consultation, and we will be happy to serve you.