Detection method of trimethylaminomethane
Tris, also known as Tris, is an important laboratory reagent. It is widely used in molecular biology, biochemistry, and other fields, but due to the uneven quality of products on the market, sometimes counterfeit and inferior trimethylaminomethane can be purchased, so it is necessary to conduct quality and purity testing. Below is a related explanation for the determination of its appearance, content, solubility, drying loss, pH value, melting point, and UV absorbance.
Trihydroxymethyl aminomethane powder
Appearance inspection is one of the more basic testing methods, usually through normal visual inspection. The sample is observed under natural light to preliminarily evaluate its appearance and ensure that there are no obvious foreign objects or impurities.
Content determination is a key step in evaluating the purity of trimethylaminomethane contained in the sample. In this process, an electric potential titrator is usually used, with the following steps:
1. Weigh 0.25g of sample and accurately weigh to 0.0001g
2. Add the sample to 80mL of water and 2-3 drops of methyl red indicator solution.
3. Titrate with standard hydrochloric acid titrant (0.1mol/L) until the solution changes from light yellow to slightly pink.
4. Record the volume V of the titration solution, using water as the blank reference, and then calculate according to the formula.
Determination of solubility
Used to evaluate the solubility of trimethylaminomethane in water. During this process, 40g of sample was added to 60mL of pure water to form a 40% aqueous solution. The solution should be clear and transparent, without any impurities.
Determination of Loss on Drying
The determination of drying loss is used to determine the moisture content in the sample, usually operated by an electric heating constant temperature blast drying oven. The steps are:
1. Firstly, determine the constant weight of the weighing bottle and heat it in an oven at 105 ± 2 ℃ for approximately 60 minutes.
2. Take out the weighing bottle, let it cool to room temperature, accurately weigh it, and repeat the operation until the weighing difference is less than 0.3mg to ensure constant weight.
3. Take 2.0g of the sample and place it in a constant weight weighing bottle. Dry for 3 hours. When removing, cover the lid tightly, place it in a dryer and let it cool. Then weigh the sample (including the weighing bottle). Calculate according to the formula for drying weight loss.
Determination of pH value
PH measurement is a method for evaluating the acidity and alkalinity of a solution of trimethylaminomethane. Generally, a pH meter is used. The measurement step is to add approximately 1.0g of the sample to 20mL of water, dissolve and shake it well, and use it for measurement. Then, a pH meter is used to calibrate and determine the pH value of the sample.
Determination of melting point
The melting point is usually measured using a melting point meter, which grinds the sample into fine powder and places it into the capillary of the melting point meter; Set the starting temperature of the melting point meter to 150 ℃ and the heating rate to 2 ℃/min. Then insert the sample capillary into the melting point meter and measure its melting point. The melting point of trimethylaminomethane should be between 168.0-172.0 ℃.
Determination of sulfate ions
To evaluate the content of sulfate ions in the sample, use a standard potassium sulfate solution, take 1g of the test sample, dissolve it in water, and make it neutral. Then add dilute hydrochloric acid, shake well, filter, and then add silver nitrate test solution. Shake well and let stand for 10 minutes, comparing the depth with the control solution. The test solution should not be deeper than the control solution.
Determination of UV absorbance
UV absorbance measurement is used to evaluate the absorbance of trimethylaminomethane at different wavelengths. It is completed using an UV spectrophotometer. 4.0g of the test sample is taken and dissolved in water to form a 40% aqueous solution. The absorbance is measured at 280nm, 290nm, and 400nm using an UV spectrophotometer.
The above methods provide effective methods for detecting the appearance, content, solubility, drying weight loss, pH value, melting point, and other aspects of trihydroxymethylaminomethane. These methods help to quickly understand the quality of trihydroxymethylaminomethane.
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