What is the concentration of GOOD's buffer tris hcl (1185-53-1) used?

Release time:

2023-10-31


Good's buffer Tris HCl (trihydroxymethyl aminomethane hydrochloride) is one of the commonly used buffers in molecular biology experiments. It can stably maintain the acidity and alkalinity of the solution within a certain pH range, ensuring the accuracy and stability of the experiment. However, different experimental requirements may require different concentrations of Tris HCl buffer, and some people are not sure how its different concentrations are formulated? Let's explore how to determine the optimal concentration of Tris HCl buffer and provide some commonly used concentration configuration methods.

Why do we need different concentrations of Tris HCl buffer?

Tris HCl buffer is suitable for many biochemical and molecular biology experiments, such as enzyme activity, preparation of compound amino acids, nucleotides, protein electrophoresis, etc. Its main function is to maintain the acidity and alkalinity of the solution within a certain pH range, thereby maintaining the stability of the reaction. These different experiments generally require different pH conditions and buffering capabilities. For example, some experiments require higher concentrations of Tris HCl buffer to maintain stability in the reaction, while others may require lower concentrations of Tris HCl buffer to avoid affecting other key components in the reaction. Therefore, determining the ideal concentration of Tris HCl is crucial for various experiments.

Determine the ideal concentration of Tris HCl

Determining the ideal concentration of Tris HCl usually requires a series of experiments, and the following is a common experimental design:

1.Determine the required pH value: Firstly, determine the pH range required for the experiment. Tris HCl has different buffering capabilities at different pH values, so you need to know which pH value the experiment runs at more stably.

2.Preliminary concentration testing: Use Tris HCl buffer of known concentration (usually 1 M) to test different concentrations of Tris HCl, usually 1 M ×、 zero point five ×、 zero point one × Wait, you can obtain these different concentrations by diluting 1 M Tris HCl.

3. Adjust pH value: Adjust Tris HCl buffer solutions of different concentrations to the desired pH value, which can be adjusted using concentrated hydrochloric acid (HCl).

4. Testing buffering performance: A series of experiments were conducted using Tris HCl buffer solutions of different concentrations, and their buffering performance was measured separately, including determining their buffering capacity at the required pH and the stability of the solution.

5.Choose the optimal concentration: Based on the experimental results, choose the ideal Tris HCl buffer concentration to achieve superior buffering performance.

Preparation method of Tris HCl buffer

Once the concentration of Tris HCl buffer is determined, the following methods can be used to configure different concentrations of Tris HCl buffer:

1 M Tris HCl (pH 7.4, 7.6, 8.0):

(1) Weigh 121.1 grams of Tris powder and place in a 1-liter container.

(2) Add approximately 800 milliliters of deionized water and thoroughly stir to dissolve.

(3) Use concentrated hydrochloric acid (HCl) to add an appropriate amount of HCl according to the desired pH value (7.4, 7.6, or 8.0) to adjust the pH.

(4) Bring the solution to a constant volume of 1 liter.

(5) After high-temperature and high-pressure sterilization, store at room temperature.

Attention: The solution should be cooled to room temperature before adjusting the pH value, as the pH value of Tris solution will change with temperature. For every 1 ℃ increase, the pH value of the solution will decrease by approximately 0.03.

1.5 M Tris HCl (pH 8.8):

(1) Weigh 181.7 grams of Tris powder and place in a 1-liter container.

(2) Add approximately 800 milliliters of deionized water and stir thoroughly to dissolve.

(3) Adjust the pH value to 8.8 using concentrated hydrochloric acid (HCl).

(4) Bring the solution to a constant volume of 1 liter.

(5) After high-temperature and high-pressure sterilization, store at room temperature.

Note: The solution should also be cooled to room temperature before adjusting the pH value.

0 × TE buffer (pH 7.4, 7.6, 8.0):

This buffer is often used in molecular biology experiments, such as for DNA dissolution.

(1) Prepare the following solution and place it in a 1-liter container: 100 mM Tris HCl Buffer (pH 7.4, 7.6, or 8.0) 100 mL, 500 mM EDTA (pH 8.0) 20 mL

(2) Add approximately 800 milliliters of deionized water to the container and mix evenly.

(3) After the solution is brought to a constant volume of 1 liter, it is sterilized under high temperature and pressure.

(4) Store at room temperature.

Good's buffer Tris HCl is a key component of molecular biology experiments, but its concentration needs to be determined based on the pH range and buffering capacity required by the experiment. As an advantageous manufacturer of biological buffering agents, Desheng can provide Tris HCl raw material powder for manufacturers to prepare and use, facilitating transportation, storage, and transfer. We also have an independent warehouse and stable supply. If you are interested, please feel free to consult us at any time!