Shelf life of HEPES buffer: How to maintain ideal performance?
HEPES (4-hydroxyethyl piperazine ethanesulfonic acid) is a commonly used buffer, widely used in biochemical and molecular biology experiments. Its excellent buffering performance and chemical stability make it an ideal buffer for experimental personnel, but how to properly store HEPES to maintain its ideal performance has become a concern for many people.
HEPES is a zwitterionic buffer with pKa ranging from 7.45 to 7.65, suitable for biochemical experiments such as cell culture and enzyme reactions. Its shelf life mainly depends on the following factors:
1. Packaging: HEPES is usually supplied in the form of white crystalline powder, and well sealed packaging can prevent the invasion of moisture and CO2 in the air. Therefore, when purchasing HEPES, attention should be paid to selecting well sealed packaging and not removing it randomly. After purchasing, seal unused HEPES in their original packaging, try to eliminate air as much as possible, and ensure good sealing.
2. Temperature and humidity: HEPES are very sensitive to temperature and humidity, and high humidity environments can cause HEPES to absorb moisture, which reduces its performance. Therefore, it is necessary to maintain dryness during storage. In addition, it should be stored at room temperature, away from high temperatures. If formulated as a solution, it needs to be stored at 4 ℃. Excessive temperature can cause its decomposition and buffer performance to fail.
3. Pollution: Do not store HEPES with other substances that are prone to chemical reactions, as they can easily cause pollution. Use clean tools and containers to handle HEPES. In addition, it is recommended not to open the same bottle of HEPES multiple times, as excessive humidity and air can repeatedly enter, leading to poor performance.
In addition, the effectiveness of HEPES can be checked by measuring the pH value of HEPES buffer using a pH meter. If it approaches a pH range of 6.8-8.2, there is no change in the buffering performance of HEPES. Additionally, it is possible to gradually adjust its pH using an acid or base with a known pH, and monitor its buffering effect to determine whether HEPES is inactive.
HEPES is a good buffer, but to maintain its ideal performance, correct storage is crucial. As a HEPES manufacturer , Desheng produces buffer products of analytical purity, with a purity of up to 99%. They are often powder and ready to use, making transportation and storage convenient. At present, there is stock supply in the warehouse, with favorable prices to meet demand in a timely manner. If you have any interest, please feel free to contact us for purchase!
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