Additives for Vacutainer - "sharp tool" for blood collection and processing
When many people seek medical treatment in hospitals, doctors often issue checklists, which include an important test called blood routine. Medical staff will judge the disease based on the patient's blood changes. However, in order to obtain accurate and reliable test results, relying on laboratory analysis is not enough. Correct collection, retention, and processing of blood samples play a crucial role.
1、 Blood sample collection
One common method is to use a vacuum tube blood collection method, which uses a bidirectional needle to pierce a vein with the help of a needle holder and insert one end into the vacuum tube. Blood automatically flows into the tube in a vacuum environment. Due to the fact that blood collection is carried out under closed conditions, the phenomenon of contamination caused by blood leakage is avoided, and it is beneficial for the transportation and storage of blood samples.
2、 Blood sample processing
Many times, improper handling of blood samples can lead to analysis errors and result in problems with the test results. Therefore, during blood processing, appropriate Anticoagulant is often selected to assist in detection according to detection requirements, so as to reduce errors. Anticoagulant refers to the control of coagulation factors by chemical means, so as to prevent blood coagulation. Generally, the requirements of anticoagulant are small dosage, high solubility, and no interference in testing experiments. The vacutainer additives commonly used in biochemical tests are as follows:
The main principle of action is to inhibit the activity of thrombin, prevent platelet aggregation and adhesion. Heparin anticoagulation is often used in biochemical testing projects and blood gas analysis. Typically, a 1.0g/L heparin solution can anticoagulate 5 ml of blood.
2. Potassium oxalate, Sodium fluoride
When potassium oxalate is exposed to calcium ions in the blood, it will generate Calcium oxalate, thus preventing blood coagulation. It has high solubility and strong anticoagulant effect. Adding potassium oxalate and Sodium fluoride together into the test tube for use can prevent Glycolysis for blood glucose detection. If Sodium fluoride is not added, the glucose content in the blood sample will drop rapidly. If Sodium fluoride is added, the blood glucose concentration can be kept for a day at 25 ℃.
3. EDTA salt
EDTA can chelate calcium ions in the blood, inactivate thrombin and interfere with the coagulation process, thus playing the role of blood anticoagulation. It is suitable for general Hematology inspection, not for coagulation test and platelet function inspection.
3、 Blood sample separation
Blood samples should be separated from serum or blood clots as soon as possible after collection, otherwise it may cause mutual conversion of components between red blood cells and serum, or affect the detection results after cell decomposition. Commonly used reagents for separating serum and plasma include anti irradiation serum separating gel, which can not only separate serum and blood clots well, but also be used in test tubes together with Anticoagulant to help test results more accurately.
Desheng Biochemical has been committed to the research, development and production of Vacutainer additives, whether it is serum separating gel product quality has been recognized by customers, and has established contacts with many countries around the world. It has opened up its own way in the market. If you have any needs for blood collection reagents, please feel free to call for consultation!
MOPS buffer is an important biochemical reagent used to maintain the acid-base balance and ion environment of tissue samples, protecting cell structure and function. Widely used in cell culture, tissue fixation, and immunohistochemical staining to improve experimental accuracy and reliability. Understanding the characteristics and application principles of MOPS buffer is crucial for biomedical research.