What operations will directly affect the effectiveness of serum separation gel? Collect lightning protection!
Separating adhesive blood vessels can meet the demand for quickly providing high-quality blood samples, and the formation of isolation layers can also make the composition of blood samples more stable. When using serum separation gel to collect blood vessels in clinical testing, some seemingly insignificant operations may affect the separation effect, and these issues are not easily detected in daily quality control and routine testing. Let's follow the editor to lighten the lightning!
Serum separating gel
1. Quality of serum separation gel
The specific gravity of the separation gel is between serum (plasma) and blood cells, so it can separate serum and blood cells. If the quality of the blood vessel separation gel is poor and the specific weight cannot meet the requirements, it is easy to see the phenomenon of the separation gel and serum (plasma) interwoven together.
2. Incomplete blood coagulation
Sometimes, after centrifugation, there may be incomplete separation of the separation gel layer and serum and blood clots, as well as the appearance of fibrin filaments in the serum. Perhaps the blood did not completely coagulate before centrifugation. Generally, blood collection vessels containing coagulants need to be placed upright for about 30 minutes, while blood collection vessels without coagulants need to be placed upright for 60-90 minutes before centrifugation.
3. Centrifugal temperature
The centrifugal temperature has a significant impact on the effectiveness of separating serum with gel promoting tubes. If the temperature exceeds the storage temperature required for the separation tube, the inert separation gel will dissolve in the serum. Not only will it cause blockage and pollution to the sample probe and reaction cup of the biochemical analyzer, but it will also have a significant impact on some biochemical measurement results.
4. Centrifuge operation
Centrifuge is an important step in the preparation of high-quality serum samples using separation gel to promote coagulation. Generally, after blood collection, the separation gel for blood collection requires immediate 180 ° gentle inversion and mixing 8-10 times, standing upright for 30 minutes until the sample has fully solidified. Centrifuge for 10 minutes using a horizontal centrifuge, and maintain the centrifugal force at 1300-2000 degrees × g. Completely separate the serum (plasma) from the blood clot by separation gel.
Desheng Biochemistry is a high-tech enterprise engaged in the research and development, production, and operation of in vitro diagnostic products. It has its own serum separation gel research and development patent, and the product quality is reliable. Welcome to consult and order!
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