How to prepare Luminol reagent? You'll know after reading it
Usually, forensic medicine uses chemical or biological techniques to collect and analyze criminal evidence, including fingerprint collection and blood identification, such as using tetramethylbenzidine to excavate fingerprints and using luminol for blood screening. The application of these reagents has fully provided corresponding clues for the occurrence of on-site accidents. Due to the presence of many novice personnel in these fields, they do not know how to prepare Luminol reagents. Below, we will focus on understanding it.
1、 Luminescence reaction mechanism
Because it is also known as luminescent ammonia, as a stable luminescent substance, it is fully utilized during criminal investigation to detect blood residue, which is why it can play a role. Mainly because it can react with iron in the blood, and under the catalysis of iron, it can quickly decompose into water and monooxygen. This way, once there are blood stains, it will quickly produce blue light. Usually, the reaction process is very sensitive, even one millionth of blood that has been left for many years can be detected, and criminal investigators can effectively distinguish and extract it.
2、 Preparation process
1. First, you need to prepare a bowl, a spoon, an appropriate amount of Luminol powder, soda powder, distilled water, hydrogen peroxide, spray bottles, etc.
2. Mix the powder with baking soda and pour into a dry bowl.
3. Add a small amount of distilled water for the first time, and add a certain amount of hydrogen peroxide to the mixed powder in the bowl for the second time. Stir thoroughly and evenly to form a solution state.
4. Pour the solution into a spray can and use a cotton cloth stained with blood as the experimental object. Spray the luminol solution onto the cloth, turn off the surrounding lights, and observe for any luminescence and color in a dark environment.
The preparation of Luminol is relatively simple. As long as the dosage is well controlled and reliable suppliers are selected, there will be almost no interference during the experiment, which can quickly help achieve the goal. As a professional manufacturer of luminescent reagents, Desheng has 6 acridine ester series and 3 Luminol series. In addition, customized services can be provided to meet customer needs to ensure customer satisfaction. Due to the R&D team constantly mastering data dynamics and strict requirements in terms of performance, fast and sensitive luminescence is the choice of most people. If you are interested, please click on the website to inquire and purchase!
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