Is anticoagulant of blood collection vessel the main reason affecting electrolyte detection?
Release time:
2023-01-12
The progress of science and technology has brought about the comprehensive development of laboratory automation, which not only improves the overall detection efficiency of medical institutions, but also significantly reduces errors in the analysis process. However, reducing errors does not mean that there are no errors. Errors in electrolyte detection often occur in clinical work. What is the matter? Some people suspect that the reason for the use of anticoagulants is really so? Here's a detailed introduction.
In fact, the error of electrolyte test results is related to a variety of factors. Anticoagulant is only one of the few reasons, not the main reason. There are also the following reasons related to it:
1、 Hemolysis of blood sample
In the process of blood collection, when the hemolysis phenomenon is caused by improper operation of the medical staff, the red blood cells are broken. At this time, the potassium ions in the EDTA anticoagulant that has been in contact with the blood flow out, and the potassium ions contained in the blood are released rapidly, which will lead to the high potassium ion concentration of the whole blood cell, leading to incorrect electrolyte detection.
2、 Specimen storage time
The blood sample was put aside for a long time, and the test was not completed in time. The exchange of substances inside and outside the blood cells in the test tube led to certain changes in the plasma composition during metabolism, which affected the test results. Generally, the longer the blood sample was kept, the greater the impact on the results. At this time, the electrolyte test was also inappropriate.
3、 Collection volume of blood sample
If the amount of blood sample collected does not reach the required content of the vacuum tube, the residual vacuum in the vacuum tube will be squeezed and contracted, causing the release of potassium in the cell and the increase of external potassium. At this time, the detection will also have problems.
4、 The anticoagulant is contaminated
The commonly used anticoagulants in clinical medical experiments are EDTA potassium salt series reagents, as well as heparin salt or sodium citrate. The anticoagulant principles of these different anticoagulants are obviously different. Generally, anticoagulant tubes such as heparin will be selected for electrolyte emergency test items, but sometimes EDTA-K2 will be used for routine inspection to achieve anticoagulant effect by chelating calcium ions. If the anticoagulant is contaminated, it will directly lead to potassium increase and abnormal test results.
There are many factors that cause the abnormality of electrolyte test results. People or inexperienced medical staff should not blindly think that it is the cause of anticoagulant for blood collection, but should analyze it according to the actual situation.
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