HEPES for cell culture media, animal husbandry

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HEPES biological buffer, the Chinese full name is 4-hydroxyethylpiperazine ethanesulfonic acid, CAS number is 7365-45-9, its role is mainly to adjust the surrounding pH value changes caused by biological metabolic wastes in the biochemical reaction environment, so as to make The biological environment is maintained within a pH range suitable for the continuation of biochemical reactions. HEPES buffers are buffered from pH 6.8 to pH 8.2 and are widely used in products such as cell culture media, animal husbandry and clinical experiments.


HEPES buffer


HEPES is commonly used in cell culture. The addition of HEPES buffer to the culture medium removes calcium ions from the 3D cell culture medium and maintains a favorable range for cell growth. The addition of HEPES to the mitochondrial cryopreservation agent can effectively protect the mitochondrial structure and maintain its function after thawing, and provide the effect of maintaining the pH of the isotonic solution.

In animal husbandry, mature sperm and eggs are removed from animals for artificial insemination in vitro. To ensure the activity of sperm and eggs in vitro, it is necessary to add a certain concentration of HEPES to the culture medium that simulates the physiological environment in vivo to stabilize the pH value in the living environment of sperm and eggs, thereby improving the survival probability and increasing the probability of artificial insemination.

Hubei Xindesheng Material Technology Co., Ltd. integrates production and research and development. The HEPES produced by our company is usually not only used in the above-mentioned applications, but also in detection reagents and biological preservation solutions. It is worth mentioning that, as a cosmetic raw material, our HEPES has been qualified for submission.