FAQ-12 common problems of blood collection tube additives Lithium Heparin

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1. What is the anticoagulation mechanism of lithium heparin?

Answer: Heparin is a mucopolysaccharide substance containing sulfate groups extracted from animal intestinal mucosa. It is a biologically active substance with a strong negative charge. Heparin has the effect of activating anti-thrombin Ⅲ and inactivating serine protease in the blood, thereby preventing the formation of thrombin, and preventing platelet aggregation and other anticoagulant effects. It affects all three stages of the coagulation process, and has anticoagulant effects both inside and outside the body, which can extend the coagulation time, prothrombin time and thrombin time.


2. Does the heparin lithium green cap blood collection tube needs to be dried in practice?

Answer: Yes, it must be completely dried. Because lithium heparin is a mucopolysaccharide substance, it is very easy to breed bacteria in an aqueous solution. If it is not dried, the product is easily deteriorated and loses its activity.


3. What is the potency of Desheng's lithium heparin?

Answer: Desheng generally provides 160IU/mg lithium heparin. In actual use, the potency of lithium heparin does not affect the use effect, but affects the dosage. Generally, we recommend adding 0.125 mg of lithium heparin (160IU / mg) per ml of blood.


4. What grade lithium heparin should be used in blood collection tubes need?

Answer: Lithium heparin in blood collection tube needs biochemical grade lithium heparin.


5. What is the recommended dosage of lithium heparin as an additive for common blood collection tubes?

Answer: The general recommended dosage is 15-25 IU per ml of blood. The recommended dosage is 20 IU/ml. The specific usage can be based on the actual situation.


Lithium Heparin Blood Collection Tube


6. What is the correct operation of the green cap anticoagulation tube after blood collection?

Answer: In order to ensure sufficient blood anticoagulation, the heparin salt test tube must be mixed upside down 5-8 times immediately upon blood collection , especially when the blood collection environment temperature is higher than 25 ℃, the blood and lithium heparin must be mixed thoroughly and timely, otherwise it may lead to Blood clotting or partial clotting.


7. Why do more than 100+ blood collection tube manufacturers in China choose lithium heparin produced by Desheng?

Answer: Desheng's lithium heparin is produced by itself, with independent intellectual property rights and the production process is completely traceable and controllable. In line with the business philosophy of ethics first and sincerity first, Desheng's lithium heparin has the characteristics of high purity, accurate potency, and precise colony control range.


8. Lithium heparin and sodium heparin are additives for green blood collection tubes, so what is the difference between the two?

Answer: There are three commonly used heparin salt: ammonium, lithium and sodium heparin. Lithium heparin is the most suitable heparin because it has the least effect on the testing of other ions. Lithium heparin basically contains no excess ions. It should not be used to test lithium blood collection tubes. Second, heparin lithium has less delay in anticoagulation than sodium heparin. Heparin is the only anticoagulant that can be used to determine pH, blood gas, electrolytes and calcium ions.


9. Why do some lithium heparin tubes have tiny clotting fragments after blood collection?

Answer: This is because the partial concentration of anticoagulant in the lithium heparin anticoagulation tube is too high, which causes some blood to enter the blood collection tube and cannot be fully mixed with lithium heparin, resulting in a small clot. This may be due to the failure to reversely mix the test tubes in time after blood collection.


10. In addition to being an additive to the green cap, can lithium heparin be used as an additive to other tubes?

Answer: Yes. It can be used in combination with sodium fluoride and separation gel. When heparin lithium is used in combination with sodium fluoride, it can be used in blood glucose tubes, adding about 20IU per ml of blood. When lithium heparin is used with the separation gel, the dosage of lithium heparin remains unchanged, and 0.8-1.2 grams of separation gel can be added to each tube.


11. What is the difference between lithium heparin as an anticoagulant and EDTA salt?



Lithium Heparin


Anticoagulation mechanism

Inactivates the action of serine protease by activating antithrombin III, thereby preventing the formation of thrombin, and preventing platelet aggregation and other anticoagulant effects

Reversible chelation with calcium ions in the blood to achieve anticoagulation


Action Stage

Multi-stage in vivo or in vitro

Endogenous or exogenous coagulation

Application Range

Suitable for erythrocyte fragility test, blood gas analysis, hematocrit test, erythrocyte sedimentation rate and general energy biochemical determination, not suitable for hemagglutination test. Excessive heparin can cause leukocyte aggregation and cannot be used for leukocyte counting. Because it can make the blood stained background light blue, it is not suitable for the classification of white blood cells

Suitable for general hematology test, not suitable for coagulation test and platelet function test, nor for determination of calcium ion, potassium ion, sodium ion, iron ion, alkaline phosphatase, creatine kinase and leucine aminopeptidase , Suitable for PCR experiments.




12. What are the storage conditions for lithium heparin?

Answer: This product can be sealed and stored in a cool and dry place, protected from light, and can not be damp. The aqueous solution of this product can be sealed and stored in a sterile state at 0-4 ℃. The maximum storage period should not exceed 7 days. It is recommended that the aqueous solution of this product is ready to use immediately, and do not store for a long time in the state of solution. Lithium heparin is forbidden to use foreign substances, abnormal smell, color and effective shelf life.