Effects of Blood Collection Tube Additive Dipotassium EDTA on Blood Cells
Dipotassium EDTA is a strong anticoagulant and is often used in vacuum blood collection tubes as an additive for blood collection tubes. It has a chelating effect on metals and can chelate metal ions in the mixture, including iron ions and calcium ions, reducing their reactivity. Because calcium ions are required in the process of blood coagulation, when the calcium ions in the blood are chelated by dipotassium EDTA, the necessary conditions for coagulation are lost, thereby achieving the effect of blood anticoagulation.
Dipotassium EDTA is a commonly used reagent in clinical routine testing items such as blood cell analysis. It is suitable for a number of hematological examinations and plays a very important role in the diagnosis, treatment and efficacy observation of clinical diseases. It has the characteristics of good anticoagulation effect and relatively little influence on the shape of blood cells, and can keep the volume and shape of red blood cells, white blood cells and platelets unchanged, and has good stability.
Due to these advantages, dipotassium EDTA is unanimously considered as an ideal anticoagulant for complete blood count and classification. Although it is an ideal anticoagulant, it should not be sloppy in use. Let's take a look at the practical application. Something to watch out for.
1. Concentration selection of dipotassium
At present, vacuum blood collection tubes containing EDTA-2K anticoagulant are widely used, and blood collection in the daily testing process is usually 0.5-2ml.
2. The ratio of blood to dipotassium anticoagulant
The ratio of blood to EDTA-2K anticoagulant is seriously out of balance, which can lead to coagulation or slight coagulation of blood samples, and even blockage of blood analyzer pipes or inaccurate analysis results.
3. Operation after blood collection
After blood collection, invert and mix 5-8 times to ensure that the anticoagulant is fully mixed with the blood, otherwise it will cause the blood to coagulate locally or form a blood clot.
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