Application and configuration method of CAPSO sodium salt
The Chinese name of CAPSO sodium salt is 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid sodium salt, and the CAS number is 102601-34-3. It is a widely used zwitterionic buffer in biochemical experiments. It is also one of Good's buffers. Its appearance is a white powder, and it has good solubility in water. Compared with other insoluble buffers, it is more convenient to use. Let's take a look at its specific applications in biochemical experiments.
Application of CAPSO sodium salt:
1. CAPSO sodium salt is non-toxic to cells and can be used as a buffer in cell culture medium;
2. Use in Western blotting and Western blotting experiments;
3. For the preparation of enzymatic reactions and detection reagents;
4. For electrophoresis and chromatographic analysis experiments;
5. For protein sequencing and identification.
Although CAPSO sodium salt is not as familiar as the common TRIS and HEPES, its application is also very broad, so what should be paid attention to when using it? How is the configuration method? Xiaobian here to let you understand thoroughly.
The configuration method of CAPSO sodium salt:
1. Prepare the required proportions of CAPSO sodium salt powder, distilled water (dH2O), stirring bar, and a large beaker;
2. Pour CAPSO sodium salt and the corresponding proportion of distilled water into the beaker;
3. Stir with a stirring bar until the CAPSO sodium salt powder dissolves;
4. Add CAPSO to adjust to the required pH value;
5. Pour distilled water (dH2O) to the final volume;
6. Filter, sterilize and sterilize to obtain the finished solution.
Precautions for the use of CAPSO sodium salt:
1. CAPSO sodium salt powder should be sealed at room temperature and stored away from light, and the prepared solution should be stored at low temperature at 4 degrees Celsius;
2. In order to maximize the buffering effect of CAPSO sodium salt, it is best to use it immediately;
3. Take precautions when using in experiments, avoid direct contact with the skin, and rinse with running water in time if it accidentally gets on the skin.
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