Difference Between Acidic, Alkaline, and Neutral Biological Buffers

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In many experiments in chemistry and biology, changes in pH can have significant negative effects, so biological buffers are required to maintain the pH balance required for the experiment and protect it from external environmental influences. A buffer solution It is made by mixing a large amount of a weak acid or base with its conjugate base or acid, and when a small amount of acid or base is added to it, its pH does not change significantly because a buffer solution blocks the acid and base neutralize each other.

Difference Between Acidic, Alkaline, and Neutral Buffers

Acidic solutions contain more hydrogen ions than hydroxide ions, and in the pH range 0 to 14, acidic buffer solutions have a pH below 7. They are usually made from a weak acid and a conjugated base (usually the sodium salt). For example glycine-hydrochloric acid buffer.

Alkaline solutions contain more hydroxide ions than hydrogen ions, are in the pH range of 0 to 14, have a pH above 7, and are usually made of a weak base and one of its salts. For example, CAPS buffers are alkaline buffers.

A neutral solution is one with a pH of 7 that is classified as neutral, such as MOPS buffers. For the convenience of memory, the editor has organized the key content of three types of buffers.

① At pH < 7, the solution is considered acidic due to the high concentration of hydrogen ions. 

② At pH > 7, the solution is considered basic due to the low concentration of hydrogen ions. 

③ At pH = 7, the solution is considered neutral because the hydrogen and hydroxide ion concentrations are the same.

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