What are the applications of HEPES and CHAPS buffers?

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HEPES and CHAPS buffers are both commonly used buffers in biochemical testing. The full chemical name of HEPES is 4-hydroxyethylpiperazine propanesulfonic acid, which is a zwitterionic buffer. It is a white powdery solid at room temperature. High water and water solubility. The chemical name of CHAPS is 3-[(3-chloroaminopropyl)dimethylammonium]-1-propanesulfonate, which is also a white powder and has good water solubility, but also hydrophobicity at the molecular level . When CHAPS is dissolved in water, the appearance of its aqueous solution will be colorless to pale yellow transparent color.

Application of HEPES buffer

Since the growth and metabolism of cells in culture can cause dramatic changes in the environmental pH of the medium, it is necessary to add an appropriate amount of biological buffer to maintain an environment in which cells can be stably cultured. HEPES buffers in the pH 6.8-pH 8.2 range, which is beneficial for cell culture. So HEPES is widely used in cell culture, tissue culture, protein purification and extraction, immunoprecipitation, cell lysis, live cell imaging and other biological and biochemical studies.

Application of CHAPS Buffer

Several studies have shown that, compared with NP-40 and Triton X-100, CHAPS can gently and effectively disrupt the nuclear membrane, solubilize membrane proteins, and disrupt protein-protein interactions (PPIs). In addition, CHAPS can preserve the native state or conformation of the protein, and can maintain the activity and function of the protein. Therefore, CHAPS is widely used in the field of biological science research, such as cell lysis and protein purification. It is important to note that when preparing any solution using CHAPS, try not to shake or agitate vigorously.

Desheng Company is a manufacturer engaged in the research, development and production of biological buffers. At present, the HEPES and CHAPS products produced are stable in performance, good in use, and have a purity of 99%. If you need to buy, welcome to inquire and order.