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How to configure TES buffer

Release time:

2022-03-30

The Chinese name of TES buffer is trimethylolmethylaminoethanesulfonic acid, CAS number: 7365-44-8. It is composed of Tris-HCL, EDTA and SDS. Therefore, it is called TES, which is a pH buffer. It has a pKa value of 7.550 (I=0,25°C). It is one of Good's buffers and can be used to make buffered solutions in the pH range of 6.8–8.2. It is one of the components of the test yolk buffer medium used for semen refrigeration and transport.

Many products on the market are TES powder, and there are few prepared solvents. Because of the consideration of transportation and storage, they are generally sold in powder form. Many people may not know how to configure and use them after buying them. Let's take a look. How is TES configured into a solution?

Configuration method of 1L TES solution:

1. Prepare materials

229.25g TES powder, 800ml dH2O (distilled water), 10N sodium hydroxide, beaker.

2. Operation steps

① Pour 800ml of dH2O (distilled water) into a large beaker;

② 229.25 grams of TES powder is poured into a large beaker and mixed with dH2O (distilled water) to dissolve;

③ Adjust the solution to the required pH value with 10N sodium hydroxide;

④ Add dH2O to make the solution volume to 1L and the configuration is complete.

There are many manufacturers selling TES buffer (trimethylolaminoethanesulfonic acid) on the market. When purchasing, be sure to keep your eyes open and buy from big manufacturers. Both use and after-sales can bring the best Serve. Hubei Xindesheng Material Technology Co., Ltd. is a professional manufacturer of biological buffers. Integrating research and development and production, there are many types of biological buffers currently produced, including TES, TRIS, HEPES, MOPS and other products. The product has stable performance and good use effect. If you have any purchase needs, please feel free to call us to consult and order!