What role does Tris buffer play in nucleic acid PCR experiments?
Release time:
2022-03-27
What is PCR
A technique that has revolutionized the field of molecular biology and beyond, the polymerase chain reaction (PCR) is used to amplify specific DNA fragments from a complex mixture of starting materials called template DNA. Sample preparation and purification protocols depend on the availability of starting materials, including sample matrix and target DNA.
PCR buffer
PCR buffer ensures optimal conditions throughout the PCR reaction. The main components of PCR buffer include magnesium chloride (MgCl2), tris-HCl and potassium chloride (KCl). MgCl2 acts as a cofactor for DNA polymerase, while tris-HCl and KCl maintain a stable pH during the reaction. PCR reactions were performed in individual tubes by mixing the above reagents and placing the tubes in a thermal cycler.
Tris buffer is commonly used in PCR experiments. It can also be used for in vitro cell culture, enzymatic analysis, and some electrophoresis applications at physiological pH. The PCR experiment buffer is generally preferably Tris buffer system; wherein the divalent cation is selected from Mg 2+, Ca 2+, Mn 2+, Cu 2+, Fe 2+, Ni 2+, Zn 2+ and Co 2+; chelating agent Selected from EGTA, EDTA and NTA. Nucleic acid PCR is currently an important detection method for viral nucleic acid detection, so what is the role of Tris in nucleic acid PCR experiments?
The role of Tris buffer in PCR experiments
In DNA electrophoresis, separation, purification or PCR amplification experiments, Tris buffer is mainly used to maintain the pH stability of the reaction system to ensure the activity of enzymes and the smooth progress of various biochemical reactions. The Tris buffer system in DNA research-related experiments is usually composed of Tris, EDTA, plus acetic acid, boric acid or glycine. PCR amplification test strip DNA or RNA reverse transcribed DNA is replicated under the catalysis of Taq enzyme.
In general, Tris is the preferred buffer in nucleic acid PCR experiments. It is recommended that you purchase high-purity Tris, EDTA or other reagents provided by regular manufacturers. As a professional TRIS buffer manufacturer, Desheng always insists on providing high-quality products and services. Our fully traceable raw materials provide you with strong support in the biological field from research and development, application expansion to production.
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