virus transport media escorts nucleic acid detection
Release time:
2022-03-26
virus transport media is an additive used in nucleic acid detection. Nucleic acid detection includes steps such as virus sample sampling, nucleic acid release, nucleic acid extraction and purification, and PCR amplification. Among them, virus transport media is used in the process of virus sample collection, transportation and preservation. A liquid medium to protect the sample.
The current virus transport media is usually used for nucleic acid detection of new crowns. Nucleic acid detection attaches great importance to timeliness and needs to be fast and accurate. But this is not absolute, because there are two types of preservation solutions - inactivated and non-inactivated. The inactivated type can quickly inactivate the virus and release viral nucleic acid for rapid viral nucleic acid detection; the non-inactivated type can protect the activity of viral RNA and protein at the same time, which can be used for viral nucleic acid detection as well as antibody and antigen detection.
Advantages of using virus transport media:
1. It can preserve samples collected from tissues or organs such as the nose and pharynx.
2. The virus transport media can be used to preserve RNA viruses and DNA viruses. In the process of preservation, the infectivity of the virus is severely weakened or even completely lost.
3. Protect nucleic acid, make DNA and RNA without obvious degradation within a few days.
4. Samples in virus transport media whose components are compatible with subsequent PCR, RT-PCR, LAMP or RT-LAMP.
5. Samples collected by multiple swabs can be saved, such as 5-mix 1, 10-mix 1, and 20-mix 1 sampling tubes.
Precautions for the use of virus transport media
The virus transport media is only for scientific research and cannot be used to preserve live viruses, nor to study the cytotoxicity of viruses.
Although the preservation solution contains virus inactivator, it cannot be ruled out that the centrifuge tube is contaminated with viruses during sampling. Therefore, operators must take appropriate protection according to the method of handling infectious samples, and must perform all operations in laboratories that meet national regulations.
Desheng has been committed to the field of in vitro diagnosis for more than ten years, and has rich experience in the field of virus transport media and blood collection tube reagents. Special reminder: operators must also take precautions accordingly. Viruses are contagious, so all the garbage in the experiment process It needs to be handled as an infectious substance.
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