How widely the application of MES buffer is, it is enough to read this article
Release time:
2022-03-13
MES Chinese name is 2-(N-morpholine)ethanesulfonic acid, it is a buffer used in biochemistry and molecular biology, it is a zwitterionic morpholine buffer, suitable for pH range of 5.5-6.7 , commonly used in cell culture media, running buffers in electrophoresis, and protein purification in chromatography. Since MES does not form complexes with most metal ions, it is used as a non-coordinating buffer in metal ion solutions. However, the application of MES is far more than that, and it is also used in many experiments. Let's take a look!
Application of MES buffer:
1. MES belonging to anion buffer is suitable for cation exchange chromatography and hydroxyapatite chromatography;
2. For gel filtration chromatography;
3. For cellulose phosphate column chromatography;
4. For hydrophobic interaction chromatography;
5. For SDS-PAGE;
6. For fluorescence microscope;
7. Suitable as non-coordinating buffer in metal ion solution;
8. It is suitable for the research of redox process;
9. For the preparation of culture medium, because it is not metabolized by bacteria and eukaryotic cells;
10. Medium for low-concentration plant cells;
11. For mammalian cell culture;
12. Suitable for most toxicity studies;
13. In vitro dissolution test for liposomes;
14. MES interacts with the peptide backbone of bovine serum albumin, resulting in net stabilization of the protein;
15. MES does not form free radical species, making it suitable for redox research;
Hubei Xindesheng Material Technology Co., Ltd. is a manufacturer engaged in the production of biological buffers. At present, there are many types of biological buffers, including MES buffer, TRIS buffer, HEPES buffer, TRIS-HCL buffer and a series of buffers It is a manufacturer integrating R&D and production. If you have purchase needs, please contact us!
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