The role of virus transport media in nucleic acid detection
Release time:
2022-03-09
virus transport media, also known as sample preservation solution, is an important consumable used in virus detection or nucleic acid detection. Nucleic acid samples require virus transport media to prevent degradation and ensure the accuracy of detection. Before that, our understanding of nucleic acids seemed to remain in the advertisements of certain nutritional products, and nucleic acids were listed as one of the nutrients, which paid the IQ tax of the masses.
The role of virus transport media in nucleic acid detection:
In the detection of new coronavirus or large-scale nucleic acid screening, the stability of nucleic acid samples is very important, so virus transport media is required. In addition, the preservation solution can also be used for the preservation of tissue and cytopathological analysis samples. During nucleic acid detection, the virus transport media is used for the preservation of throat swabs, nasal swabs or tissue samples from specific parts after sampling, and the stored samples can be used for subsequent clinical experiments such as nucleic acid extraction or purification.
Principle of nucleic acid detection:
The principle of detection is simply to say that the patient's throat swab is detected to detect the presence or absence of the genetic material (specific nucleic acid fragments) of the virus. Nucleic acid detection is carried out by RT-qPCR (real-time quantitative PCR), including multiple operations such as reverse transcription and fluorescence quantitative amplification.
Specifically, collect the patient's throat swab sample, extract RNA (ie, the genetic material of the new coronavirus), add enzymes, primers, four nucleotides, fluorescent dyes and other reagents, and perform DNA sequence amplification in the PCR machine. If there is virus in the tissue sample, then the synthesis reaction can proceed, DNA can be synthesized, fluorescent dye can be bound to the newly synthesized DNA to excite fluorescence, and the fluorescence can be observed by the instrument; on the contrary, if there is no virus, the primer will be Without finding a binding site, DNA synthesis cannot proceed and fluorescence cannot be observed. This is the principle of nucleic acid detection of viruses.
It should be noted that the protective effect of virus transport media on nucleic acid samples is temporary. If the time is too long, the nucleic acid of the samples in the sampling tube may still be degraded. Desheng's inactivated and non-inactivated preservation solutions can effectively preserve samples for one week and 48 hours. It is recommended to use a kit for nucleic acid extraction as soon as possible after sampling, and timely detection.
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