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TOPS reagent sarcosine oxidase method for determination of creatinine

Release time:

2021-12-30

TOPS reagent is a kind of color reagent commonly used in biochemical detection. It is one of the new Trinder's reagents. It is commonly used to detect blood sugar, uric acid, creatinine and other biochemical indicators. Here we introduce the creatine oxidase method using TOPS reagent. Creatinine method.

Advantages of TOPS reagent:

TOPS reagent is also known as ESPMT reagent, and its chemical name is N-ethyl-N-sulfopropyl-3-methylaniline sodium salt. The functional group is modified on the basis of the traditional Trinder's reagent, and the stability of the reagent is improved. The introduced propanesulfonic acid group also enables the TOPS reagent to be dissolved in the aqueous solution smoothly. Moreover, the color-developing product of the TOPS reagent has a relatively high absorption wavelength of 550nm, the product solution is purple-red, the molar absorbance is large, and the detection sensitivity is high.

TOPS reagent to detect creatinine

Significance of creatinine content:

Serum and urine contain creatinine, which is a commonly used indicator of kidney function and a mandatory item for health examinations. Creatinine (Cre), whose chemical formula is C4H7N3O, is a product of muscle metabolism in the human body and is mainly filtered out by the glomerulus. Every 20 grams of muscle metabolism produces 1 mg of creatinine.

The principle of TOPS reagent SOX method to determine creatinine:

SOX refers to sarcosine oxidase, which uses TOPS as a substrate for the determination of creatinine content by the sarcosine oxidase method. Creatinine is hydrolyzed into creatine under the catalysis of creatinase (creatinine iminohydrolase), and creatine is hydrolyzed into creatine and urea under the catalysis of creatinase CRH (creatine amidinohydrolase), and then SOX is used to remove creatine. Acid oxidizes to glycine, formaldehyde and hydrogen peroxide. The generated hydrogen peroxide can be measured with TOPS and 4-AAP in the presence of peroxidase. The content of the generated color product is proportional to the concentration of creatinine, and the absorbance measured with a spectrophotometer is proportional.

Using TOPS as a chromogen substrate to determine creatinine is a commonly used method. Since sarcosine and urea are produced during the process, it can also be measured by urea detection, but the sensitivity is not as good as the sarcosine oxidase method. Desheng can provide the chromogen TOPS, creatinase, sarcosine oxidase, buffers, etc. required for creatinine detection.