virus transport media and false virus detection experiment
Now that the delta variant of the new crown virus delta strain is spreading on a large scale, there are more and more virus detection and research experiments all over the world. Recently, some experimenters reported using virus transport media to treat fake virus to simulate virus detection, but they could not get the results. Why?
The reason is due to the incomplete understanding of the principle of the virus transport media and the fake virus. Here is a brief introduction to the characteristics of the virus transport media and the pseudovirus.
Features of virus transport media
It is also called sample preservation solution VTM, which is divided into two types, including inactivated virus transport media (generally referred to as MTM) and non-inactivated virus transport media (abbreviated as UTM, sometimes including VTM). The inactivated type can quickly kill the virus, prevent secondary infection, and is safer to use; the non-inactivated preservation solution retains the integrity of the virus and can be used for various research and detection experiments of the virus.
Both types of preservation solutions can be used for nucleic acid detection of viruses, but it is worth noting that the inactivated preservation solution is not suitable for the detection of pseudoviruses, which is related to the characteristics of pseudoviruses.
Features of fake virus:
The name is pseudovirus, but it is not a fake virus, but a special kind of virus: non-viral DNA is wrapped into microbial particles with the same protein shell as the virus. One retrovirus can integrate another. The envelope glycoproteins of different types of viruses form an envelope with foreign viruses, and the genome retains the characteristics of the retrovirus itself. It also becomes a pseudovirus, a retrovirus, in the form of single-stranded RNA, this type of virus mainly infects fungi and invertebrates. Simply put, a pseudovirus is a virus in which the nucleic acid of one virus is replaced by the RNA of another virus.
The pseudovirus of the new coronavirus refers to the virus that retains the protein of the new coronavirus, and its nucleic acid is replaced by the RNA of another virus. The inactivated preservation solution is to lyse the protein coat of the virus while retaining the nucleic acid, that is, the new coronavirus protein of the pseudovirus that has been lysed. The retained nucleic acid is not from the new coronavirus, so naturally it cannot be detected. In this case, non-inactivated preservation solution should be used and protein epitope detection should be used. Desheng is a virus transport media manufacturer and can supply sufficient stock of preservation solution.
In chemiluminescence analysis, the luminescence intensity of acridine ester is influenced by various factors, such as reaction medium, temperature, time, and excitation light source energy. To achieve good detection results, it is necessary to comprehensively consider and optimize these factors. Meanwhile, attention should be paid to controlling and standardizing experimental conditions to ensure accurate and reliable results. Thoroughly studying these influencing factors will help promote the development of chemiluminescence analysis methods.