What drugs affect the detection of sarcosine oxidase method?

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Creatinine (Cr) in blood is composed of two types: exogenous and endogenous. Cr is mainly filtered out of the body by the glomerulus, and the renal tubules are basically not reabsorbed and the amount of excretion is small. When the intake of exogenous creatinine is stable, the concentration in the blood depends on the glomerular filtration capacity (GFR). When the renal parenchyma is damaged and GFR decreases to the critical point, the blood Cr concentration will increase significantly, so the determination Serum creatinine concentration can be used as an indicator of GFR impairment. Sensitivity is better than blood urea (Urea), but it is not an indicator of early diagnosis.


Enzymatic detection and influence of drugs Enzymatic methods are divided into muscle tincture amide hydrolase, sarcosine oxidase method, and deiminase method based on different principles. Due to the different specific measurement principles and the same measurement principle, the source, purity, specific activity and specificity of the selected tool enzymes are different, and there are different degrees of interference between different detection methods. The sarcosine oxidase method has been applied late in clinical application, and has the advantages of simple operation, strong specificity, wide linear range, and strong anti-interference ability. It has a wide range of clinical applications and is currently one of the main methods for the detection of muscle tincture. Next, let's take a look at what drugs affect the detection of sarcosine oxidase?

Principle of sarcosine oxidase method

Calcium dobesilate

Calcium dobesilate is used as a vasoprotective agent to improve microcirculation. It is often used to treat diabetic retinopathy, diabetic nephropathy, chronic renal failure and coronary heart disease caused by other causes. The manual of the sarcosine oxidase method clearly mentions that The endogenous metabolites of calcium benzenesulfonate can inhibit the chromogenic phenol oxidation and color development during the sarcosine oxidase reaction process, which will cause the false reduction of the standard recovery of 44.2umolL. creatinine. In view of the interference of creatinine detection by creatine oxidase method by calcium dobesilate, it is highly reminded that doctors should pay attention to whether the patient is taking calcium dobesilate when sending blood creatinine to avoid false bias in blood creatinine test results. Low error.



Ethylphensulfonate has a negative interference on the determination of creatinine by the sarcosine oxidase method, which may be due to the strong reducing property of Ethylphensulfon. In the peroxidase (POD) reaction, sulfacetamide will consume the H2O2 in the POD reaction and produce a colorless product, which will cause the sulfacetamide to negatively interfere with the detection based on the Trider reaction. In view of the interference caused by sulfacetamide in the detection of serum CREA, it is recommended that those who use phenethylamine should be informed of the doctor's use of the drug during the renal function test. If necessary, the blood concentration can be tested or the body can be tested. Relevant tests are performed after the drug is completely metabolized