Why use PIPES buffer and its advantages

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What is the PIPES buffer

PIPES buffer (CAS 5625-37-6), the full chemical name is 1,4-piperazine diethane sulfonic acid, is a zwitterionic biological buffer. It is a white powder solid at room temperature. The solubility of PIPES buffer in water is not very high (1 g/L (100°C). It becomes soluble by mixing equimolar sodium salt solutions to titrate to the appropriate pH value, such as PIPES disodium salt buffer (CAS 76836-02-7) and PIPES sesquisodium salt (CAS 100037-69-2).


Application of PIPES buffer

PIPES Buffer is widely used in cell culture, chromatography, cosmetics, diagnostic testing, protein purification and electron microscopy research. PIPES buffer is similar to MES buffer and MOPS buffer. The Good's buffer lacks the ability to form complexes with most metal ions, so it is recommended to be used as a non-coordinating buffer in metal ion solutions. It can be applied to in vitro cell culture media; in the study of the determination of lactic acid in plasma by the lactate oxidase method, it is found that PIPES can be stable for 172 hours at 37°C, which is better than Tris for 72 hours.


Advantages of PIPES buffer

1. Use NaOH-PIPES buffered glutaraldehyde solution in the ultrastructure of various tissues such as human skin, human hypertrophic scars and mollusk neuron tissue. It can observe more details between organelles and tissues. The consistency of the cytoplasm is more uniform.

2. Compared with NaOH-PIPES buffer, microfilaments and microtubules are more common in PIPES tissues. It shows that PIPES Buffer is comparable to living cells in culture.

3. It can induce the polymerization of purified tubulin.

4. PIPES can meet the requirement of extremely low complexation of divalent cations when adenosine diphosphate (ADP) is bound to the relaxed fan-forming muscle fiber cells.