What are the plate and tube types in the chemiluminescence experiment of acridinium ester
Release time:
2021-02-23
At present, the domestic chemiluminescence immunoassay after rapid development has gradually become the mainstream in vitro diagnostic detection method. The chemiluminescence reagents are mainly based on luminol, alkaline phosphatase and Acridine ester systems. According to different chemiluminescence instruments, it is divided into tubular chemiluminescence and plate chemiluminescence.
Suitable substrates for plate and tube chemiluminescence:
1. The two kinds of labeling are similar in principle. Tubular chemiluminescence is also called magnetic particle (magnetic bead) chemiluminescence. The labeling can be applied to acridine esters, isoluminol flash, alkaline phosphatase glow and Electrochemiluminescence of ruthenium terpyridine;
2. Plate chemiluminescence is basically also suitable, but it is mainly used for horseradish peroxidase HRP to catalyze luminol flash.
Luminol and acridine esters are used for plate and tube chemiluminescence
The difference between plate type and tube type chemiluminescence equipment:
1. The main difference lies in the separation means. The plate type usually uses a 96-well plate, which can use a separate measuring instrument, external pre-processing equipment, or a plate-type integrated automatic model;
2. Magnetic particle chemiluminescence uses magnetic particles (magnetic beads) to label antibodies and then separate them in a magnetic field. The equipment adopts a fully automatic tube type.
Plate and tube chemiluminescence sensitivity:
In terms of test results, the plate-type precision is 5%-7%, and the linear range is 100-500 times. It takes about 1.5 hours for the batch operation to produce results. Magnetic bead chemiluminescence has higher precision, with a variation of 1%-2%, and a wide linear range of one thousand to ten thousand times. It only takes 20 to 30 minutes to collect a sample and test results, and it can support emergency temporary insertion specimen. However, the plate type uses less raw materials and low reagent cost.
Application scenarios of plate and tube chemiluminescence:
In terms of application, the magnetic bead chemiluminescence detection field is used for clinical diagnosis in tertiary and secondary hospitals. Advantageous items include endocrine hormones, microproteins, cardiovascular products, and small molecules adopt competitive methods. The advantages are obvious, and tumor markers are needed. For content tracking. Plate chemiluminescence detection is usually used in a large number of physical examinations, preliminary screening in small hospitals, preoperative safety testing, etc., items that do not require high quantification, tumor markers and other macromolecular sandwich methods, and items that do not require high mutations.
In summary, plate-type and tube-type chemiluminescence have their own advantages in different application scenarios. Therefore, Desheng has also developed and produced two series of chemiluminescence reagents, luminol and acridine esters, to meet the needs of different companies. The testing needs.
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