Advantages of inactivated virus transport media in nucleic acid detection
On January 21, Commissioner Wang Bin of the CDC of the National Health Commission stated at a press conference of the Joint Prevention and Control Mechanism of the State Council that those returning home during the Spring Festival must have a nucleic acid negative test certificate within 7 days before they can return. At the same time, after these returnees go back, the local grassroots government must conduct grid management on these people. Returnees refer to those who have returned to rural areas from other places. They mainly include those who have returned to their hometowns across provinces and those who come from cities in the middle and high-risk areas within the province.
The testing agency must feed back the test results within 12 hours after receiving the sample; if it is said that they must return to their hometown, the returnees must have a negative nucleic acid test within 7 days to return home, which will inevitably increase the testing pressure of medical staff In the process of nucleic acid detection, safety has also become extremely important. We usually use inactivated virus preservation solutions to preserve nucleic acids. It is not only because it does not need to retain virus activity, but also because it kills the virus without affecting subsequent virus nucleic acid detection. Next, let's talk about the advantages of inactivated virus preservation solution in nucleic acid detection.
1. Reduce RNA degradation and cause false negatives
The storage conditions of nucleic acid samples are harsh, RNA is easy to be lysed, and can only be stored for 24 hours at 4°C. RNA will be released if the transport process is too long
The phenomenon of premature degradation has led to an increase in false negatives. The inactivated virus transport media is a protective transport medium for the virus sample in the sampling tube, which prevents the virus from being detected in time after the virus is sampled and the virus is degraded during the inspection process, and to avoid false negatives caused by the virus degrading in advance during the test.
2. Reduce the infection of nucleic acid extraction process
Because the inactivated virus transport media is highly efficient to inactivate the virus, it is safer than the non-inactivated preservation solution in sampling and detection and processing, and will not cause secondary infection, so the operating requirements and experimental environment requirements are slightly lower . It is more advantageous for the efficiency of rapid nucleic acid detection and the calculation of detection coverage.
3. Easy to store, no degradation
The inactivated virus transport media can be stored and transported at room temperature for 1 week without degradation. After extraction according to most commercial kits, the DNA/RNA obtained is of good quality and high yield, and can complete various genetic testing and analysis experiments. Such as PCR, qPCR, etc.
4. Suitable for multiple virus detection
It is suitable for the collection, preservation and transportation of common virus samples such as new coronavirus, influenza virus, hand, foot and mouth virus. It can collect throat swabs and nasal test samples or tissue samples of specific parts. The stored samples can be used for subsequent clinical trials such as nucleic acid extraction or purification.
At present, many manufacturers of Desheng virus preservation solution are continuously and steadily repurchasing. In recent days, the sales volume has also been steadily increasing. I believe that there will be more in the later period. Desheng is very confident in its products, not only of high quality, but also Price concessions and professional after-sales technical guidance are precisely the reasons why new and old customers love and trust.
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