HRP or POD substrate chromogenic substrate TOPS for creatinine detection
The new Trinder's reagent TOPS, its full name is N-ethyl-N-(3-sulfopropyl)-3-methylaniline sodium salt, is a commonly used color reagent in biochemical kits, and is used as peroxidase HRP in enzymatic photometry Or POD substrate for creatinine detection in kits or biochemical tests.
The substance to be tested generates hydrogen peroxide H2O2 under the action of enzymes. In the presence of 4-aminoantipyrine 4-AAP, peroxidase POD or HRP, the chromogen substrate can generate red quinone imine compounds Reaction.
Chromogenic substrate TOPS for creatinine detection
Starting from the initial use of phenol, many types of chromogen substrates have now been developed. The items used in clinical biochemical testing include: glucose GLU, triglyceride GT, cholesterol CHOL, uric acid UA, high-density lipoprotein HDL, low density lipoprotein LDL, etc. The detection of creatinine in the renal function series uses TOPS as a chromogenic substrate.
The principle of TOPS for the detection of renal function and creatinine:
The method of detecting creatinine by TOPS belongs to the enzymatic photometric method. The detection principle is: creatinase hydrolyzes creatinine into creatine; creatinase hydrolyzes creatine to form creatine; sarcosine oxidase oxidizes creatine to produce hydrogen peroxide. In the presence of peroxidase, hydrogen peroxide reacts with 4-aminoantipyrine (4-AAP) and TOPS (ESPMT) or HTIB to produce quinone imines, which are colored products. The color intensity can be measured by absorbance, which is directly proportional to the creatinine concentration.
In the experiment, the standard sample tube for collecting the sample can be a vacuum blood collection tube, and the anticoagulant can be heparin or EDTA dipotassium or tripotassium produced by us. The serum or plasma is separated within 1 hour and tested immediately. Store at : 4℃- 8 ℃ stable for 7 days -20 ℃ stable for 3 months , -70 ℃ stable for a long time.
The chromogen substrate TOPS and POD or HRP enzyme preparations cannot be placed in the air for a long time. They need to be prepared for immediate use or refrigerated. The TOPS or other types of chromogen substrates produced by Desheng are all powder reagents and sealed. It can be stored for a long time, and it is not easy to deteriorate and is convenient for storage.
MOPS buffer is an important biochemical reagent used to maintain the acid-base balance and ion environment of tissue samples, protecting cell structure and function. Widely used in cell culture, tissue fixation, and immunohistochemical staining to improve experimental accuracy and reliability. Understanding the characteristics and application principles of MOPS buffer is crucial for biomedical research.