What is the difference between TBS buffer and PBS buffer

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TBS or Tris base buffer and PBS buffer are some of the more common buffer solutions, which are the basic pH buffer and buffer salt solution prepared by tris (tromethamine), and the phosphate buffer salt solution. , What is the difference between them in application?


In biochemical experiments, TBS and PBS buffers are widely used. Their functions are to adjust the pH of the reaction system, but they have their own differences. This year, TBS has been used more and more, and it has a tendency to exceed that of phosphate buffer.


TBS buffer Tris base buffer material

PBS has been used relatively frequently in the past. Phosphate buffer saline is generally used as a solvent to dissolve and protect the reagent. In the past, it was the most widely used buffer in biochemical research. The main components were Na2HPO4, KH2PO4, NaCl and KCl, and the buffer range was very wide. The main function of NaCl and KCl was to increase the concentration of salt ions.


PBS phosphate buffered saline solution can dilute general active biological agents, such as diluted serum. The reason is that it has a salt balance and an adjustable pH buffering effect, but distilled water does not have a salt balance effect, which will destroy the structure and biological characteristics of biological proteins; and physiological saline does not have the effect of adjusting pH, which is effective for complete, Active substances cannot be guaranteed to participate in biological reactions under optimal conditions, so PBS is the first choice. Sometimes higher PBS needs to add more ingredients to the balance buffer to maintain the best conditions to ensure that the biologically active substances maintain their most complete characteristics.


However, in the solution system with metal ions, the phosphoric acid system will associate with common Ca ions, Mg ions and heavy metal ions to form precipitates. Tris buffer has been used in SDS-polyacrylamide gel electrophoresis, and phosphate is rarely used. TBS adds NaCl, detergent, etc. on the basis of Tris base buffer, and the proportion of Tris in the two is also different.


TBS is Tris-HCl buffered saline (Tris Buffered, Saline) or buffered saline. The preparation method is: first dissolve NaCl (3.5-9g, 0.15mol/L) with redistilled water, then add 100mL of 0.5mol/L Tris-HCl buffer, and finally add water to make the volume to 1L, shake well to make the final Tris concentration 0.05mol/L. TBS is usually used together with the detergent Triton X-100 (non-ionic surfactant), which is often used for rinsing specimens in immunoenzyme technology.


The main raw material for the production of TBS or Tris base buffer is Tris. Desheng is a manufacturer of raw materials and also provides a variety of biological buffer raw materials for biochemical research.