D-amino acid oxidase (DAAO) application in life

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D-amino acid oxidase (DAAO, EC1.4.3.3) is a typical flavin protease with flavin adenine (FAD) as a prosthetic group, which can oxidize the amino group of D-amino acid to generate corresponding keto acid and ammonia. DAAO has been widely used in biotechnology enzyme catalysis, specifically in the following aspects:


1. Food quality inspection

DAAO enables microorganisms, especially yeast, to use D-amino acids for growth, and D-amino acids play an important role in the formation of bacterial cell walls. It is a component of peptidoglycan and muramic acid, especially D-alanine , Is one of the components of the bacterial cell wall. The presence of D-amino acids in food is a sign of bacterial contamination. Because DAAO has a wide range of substrate specificities, the determination of the overall content of D-amino acids in foods and beverages can be used as an important basis for food quality testing.


2. Treatment of mental illness and cancer

The important role of D-amino acid in metabolism has now been confirmed, that is, its changes in brain tissue, serum conditions and spinal fluid content reflect various pathological changes. Some mental illnesses, such as schizophrenia, Alzheimer's disease and Parkinson's disease can cause large changes in the content of some D-amino acids in serum, white matter, gray matter and spinal fluid. Therefore, the use of DAAO to detect the content of D-amino acid and determine the content of DAAO enzyme in the body can play an important role in early disease diagnosis and disease control.


DAAO also has broad application prospects in cancer diagnosis and treatment. Studies have shown that intravenous injection of D-amino acid preparations into cancer patients can improve nutritional status and inhibit cancer cell growth. Injection of D-proline (the most suitable substrate of pkDAAO) will induce DAAO enzyme action and significantly inhibit the growth of cancer cells. The reason is that DAAO increases the production of hydrogen peroxide in cancer cells and generates other oxidative metabolism The product does not affect the metabolism of healthy cells.