Expert ACS and ACOD enzyme preparations for breaking down fat

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Fat is the highest energy substance among the three nutrients. It stores and provides energy for the body's life activities. It is precious in the age of food scarcity, but now it seems to be the enemy of human health. The metabolism of fat in the body is mainly catalyzed by acetyl-CoA synthase ACS and acetyl-CoA oxidase ACOD.


Fat is usually called triacylglycerol or triglyceride (fatty acid glyceride), which becomes free fatty acid after digestion and hydrolysis. In the presence of Coenzyme A (CoA-SH), magnesium and consuming a small amount of energy ATP, ACS can activate fatty acids and transform them into fatty acyl-CoA. The pyrophosphate generated during the reaction is quickly hydrolyzed by intracellular pyrophosphatase, and the reaction will not proceed in reverse.


How the enzyme ACS and ACOD break down fat step by step


The water solubility of fatty acyl-CoA formed by fatty acid activation increases. The secondary process is completed in the cell fluid, and then the fatty acyl-CoA is transported through the mitochondria through carnitine. After the acyl-CoA enters the display substrate, the acetyl-CoA oxidase (dehydrogenase) ACOD will remove one hydrogen atom from each of its α and β carbon atoms to generate enoyl CoA, and the removed 2H is accepted by the FAD to generate FADH. Then hydratase catalyzes the addition of water to generate β-hydroxyacyl CoA, ACOD then dehydrogenates it to ketoacyl CoA, and finally generates acetyl CoA and acyl CoA with two fewer carbon atoms than the original one under the action of thiolase. molecular.


The above process is one of the steps for ACOD to decompose fatty acids. In summary, ACOD catalyzes the dehydrogenation, water addition, dehydrogenation and thiolysis of fatty acyl CoA to reduce two carbon atoms and generate 1 acetyl CoA. This process can be repeated repeatedly, continuously shortening fatty acids, cutting off two carbon atoms each time to generate acetyl CoA, and synthesize energy ATP. Eventually a long-chain fatty acid becomes multiple acetyl CoA and synthesizes a large amount of ATP.


It can be seen from the above that acetyl-Coenzyme A synthetase ACS activates fat to become acyl-CoA with increased water solubility, and acetyl-Coenzyme A oxidase (with other enzymes) continuously deoxidizes fat after activation It is broken down into smaller acetyl CoA. After acetyl CoA enters the tricarboxylic acid cycle, it is completely oxidized into CO2 and water, and fat is completely decomposed.


Enzyme preparations like ACS and ACOD are used in a wide range of applications, including testing, food, pharmaceuticals and many other fields. Desheng's ACS and ACOD enzyme preparations are mainly used for biochemical testing and have outstanding performance.