Application of S-adenosylmethionine synthase in medicine
S-adenosylmethionine (often abbreviated as SAM) is a physiologically active molecule that is widely present in all tissues and body fluids, whether it is prokaryotic or eukaryotic cells, S-adenosylmethionine is extremely Important metabolites. In organisms, SAM is synthesized by S-adenosylmethionine synthetase (SAM synthetase) catalyzed by the substrate L-methionine and ATP, which is the only way to produce SAM currently known in the body. Therefore, the research on SAM synthetase is particularly important. It has a great application in our medical diseases.
1. Treatment of liver and gallbladder diseases
There are many transmethylases in the liver cell fluid and microsomes. Compounds containing hydroxyl, sulfhydryl or amino groups need to undergo methylation reaction. The methyl donor is SAM. Clinically, SAM can maintain the normal function of the liver, treat many chronic liver diseases (including alcohol or non-alcohol-induced cirrhosis, estrogen-induced or other forms of cholestasis), and can reverse many drugs or compounds (such as alcohol, paracetamol, Steroids, metallic lead, etc.) cause liver poisoning. At the same time, it has obvious therapeutic effects on various hepatitis and liver failure.
2. Treat mental depression
It can increase the levels of 5'-hydroxytryptamine and 5'-oxindole acetic acid in the brain, and is effective for various depressions, especially postpartum depression. Moreover, compared with other similar drugs, it has the advantages of quick effect, no addiction, few adverse reactions, and good tolerance. Usually, the symptoms will be significantly improved within the SAM week. The combined application of SAM and conventional antidepressants, such as mianserin, clomipramine, imipramine, etc., has a faster effect than the single drug.
3. Treat arthritis
SAM also has the functions of anti-inflammatory, relieving pain and helping tissue regeneration. It can promote the formation of cartilage tissue and the healing of damage, maintain the health of bones and joints, and has significant effects on various arthritis and joint injuries. Its curative effect on osteoarthritis is equivalent to that of ibuprofen and naproxen, but it has fewer side effects and quicker effects. Compared with nonsteroidal anti-inflammatory drugs, SAM neither inhibits prostaglandin synthesis nor affects the gastrointestinal tract.
4. Treatment of fibromyalgia
Primary fibromyalgia is a chronic non-articular rheumatism. The symptoms are multiple pains and stiffness throughout the body, usually accompanied by depression. After using SAM, the number of painful sites is reduced, and symptoms such as bad mood and body stiffness are improved. In addition, SAM also has the effects of lowering blood lipids, preventing Alzheimer's disease, and anti-aging.
HEPES, as a zwitterionic buffer, increases the osmotic pressure of the cell culture system by increasing the concentration of solution ions, maintaining normal cell morphology and function, and improving cell survival rate. Widely used in cell culture, especially under specific conditions such as tumor cell culture, it is crucial to maintain cell growth and function.