What you must know about biological buffer MOPS (CAS1132-61-2)
Release time:
2020-11-08
Buffer solution is usually a solution composed of "weak acid and its conjugate base" or "weak base and its conjugate acid" buffer pair, which can slow down pH changes when a certain amount of other substances are added. 3-(N-morpholino)propanesulfonic acid, MOPS for short, is a biological buffering agent, which plays a very important role in biological experiments. Many people don’t know the specific application and configuration of MOPS, let alone its interaction with other biological buffers. What is the difference between the buffer range of the agent, Desheng will briefly introduce it today.
The MOPS buffer range is between 6.5 and 7.9, which is suitable for electron transfer and phosphorylation studies of chloroplast thin-layer preparation; it can be formulated into a variety of agar media and used as a non-toxic buffer in the minimal medium for Streptomyces coryneformis Cultivation and production of cephalosporins; it can be used as a component of the electrolyte system of two-dimensional gel electrophoresis and isoelectric focusing (IEF); it can also be used in Northern hybridization as a buffer for RNA separation and membrane transfer. The comparison between MOPS buffer range and other common buffers is shown in the following table:
|
Buffer |
pKa/25°C |
Buffer range |
|
MOPS |
7.2 |
6.5-7.9 |
|
Tris |
8.1 |
7.0-9.1 |
|
Bicine |
8.3 |
7.6-9.0 |
|
TAPS |
8.4 |
7.7-9.1 |
|
MOPSO |
6.9 |
6.2-7.6 |
|
BES |
7.1 |
6.4-7.8 |
|
HEPES |
7.5 |
6.8-8.2 |
|
CAPS |
10.4 |
9.7-11.1 |
The buffer range of several common buffers
The following describes the preparation method of commonly used 10×MOPS Buffer, as shown below
1. Weigh 41.8g MOPS and place it in a 1L beaker;
2. Add about 700mL DEPC treated water, stir to dissolve;
3. Use 2N NaOH to adjust the pH to 7.0;
4. Add the following reagents to the solution: 20mL 1M NaOAc (DEPC treatment), 20mL 0.5M EDTA (pH 8.0) (DEPC treatment)
5. Use DEPC treated water to dilute the solution to 1L;
6. Filter to remove impurities with 0.45μm filter membrane;
7. Store at room temperature and avoid light.
Desheng MOPS outer packaging
The purity of MOPS produced by Desheng is greater than 99%, the water solubility is good, and the production process is stable. However, you should also pay attention to it in use. If the amount of use each time is small, you can use it after appropriate packaging. The buffer will easily change when exposed to light. Yellow and light yellow can be used completely, but it is not suitable to use after the color is too dark. Attention should be paid during use. For safety and health, please wear laboratory clothes and disposable gloves for operation. If you accidentally contact your eyes, rinse with plenty of water and seek medical attention immediately.
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