1、 The "fragility" dilemma of chemiluminescence probes

Acridinium Ester, as a highly sensitive chemiluminescent marker, has become a commonly used in in vitro diagnostic reagents due to its advantages of no catalyst required luminescence, low background signal, and rapid response in cutting-edge fields such as immunoassays, molecular diagnostics, and biosensing. However, its inherent chemical instability has long plagued research and development personnel as well as production enterprises.

acridine esters powder

In traditional cognition, acridine esters are highly sensitive to changes in water, light, heat, and pH, and are prone to hydrolysis or spontaneous luminescence, leading to loss of activity. Therefore, most laboratories adopt a "ready to use" strategy, which is not only cumbersome to operate, but also increases the risk of batch differences, seriously affecting the repeatability and accuracy of test results. How to achieve long-term stable storage of acridine esters while also considering ease of use has become a key bottleneck in improving the performance of diagnostic reagents.

2、 Solvent selection DMF: can achieve good stability

In recent years, with the in-depth research of formulation technology and solvent engineering, a suitable preservation scheme has emerged - dissolving acridine ester in dimethylformamide (DMF) and storing it under low temperature conditions can achieve stability for up to one year, and support repeated freeze-thaw cycles without significant loss of luminescent activity.

The core of this storage method lies in the physicochemical properties of DMF. As a highly polar non proton solvent, DMF not only has excellent solubility for acridine esters, but more importantly, it can effectively inhibit the invasion of water molecules, form a stable solvation layer, and protect the active ester bonds in acridine ester molecules from hydrolysis attacks. Meanwhile, the high boiling point and low volatility of DMF make it less prone to phase separation or crystallization during repeated freeze-thaw cycles, thereby maintaining the uniformity of the solution.

3、 Behind Stability: Scientific Craftsmanship and Detail Control

To achieve this breakthrough, it not only depends on the selection of solvents, but also on rigorous preparation processes and storage standards. For example, the solution preparation process needs to be carried out in a dark environment, using brown glass bottles or aluminum foil to wrap the container to prevent photodegradation. In addition, the packaging strategy is also crucial. It is recommended to use small volume single use packaging (such as 50-100 μ L/tube) to reduce the risk of contamination caused by repeated opening and closing. When freezing, the temperature should be quickly lowered to below -20 ℃ to avoid slow crystallization and damage to the molecular structure; When thawing, it is recommended to naturally melt at room temperature to avoid severe shaking. This series of operations may seem simple, but they are actually interconnected, and any negligence in any link can lead to a significant reduction in stability.

4、 Choose reliable partners to make stability within reach

However, not all acridine ester raw materials can easily achieve this ideal state. Small differences in molecular structure, purity level, and impurity content can all affect its solubility and long-term stability in DMF. Therefore, choosing a supplier with high-purity raw material synthesis capabilities and stable formulation technical support has become the key to the success of the enterprise.

Hubei Xindesheng Material Technology Co., Ltd. focuses on the research and production of chemiluminescence reagents. Its acridine ester series products are known for their high purity, strong batch stability, and excellent solubility. The company provides customized DMF solution preparation services, accompanied by complete storage and usage guidelines, to help enterprises quickly achieve stable applications.