In biochemical testing, can samples with hemolysis be used for testing?
I used to think that as long as you memorize various theories and professional knowledge, you can do a good job of biochemical testing, but later discovered that it is far from simple. Chairman Mao said it well: Practice is the only criterion for testing truth. We know that hemolyzed samples are usually rejected and cannot be used for biochemical testing. So is this absolute? It's better to know it from practice.
In fact, hemolyzed samples are not completely incapable of biochemical testing. This problem still needs actual verification. Non-professionals usually think that biochemical tests are all kinds of pipettes with a full set of glass test tubes, which will show colorful colors during experiments. In fact, when I went to the biochemical laboratory, I discovered that the biochemical test has been highly automated. The most frequent repetitions every day are code scanning, numbering, centrifugation, specimen loading, specimen loading, specimen archiving, and so on.
Regarding the detection of hemolysis samples, it has been learned through various investigations: hemolysis samples are a type of unqualified specimens, because they will affect the accuracy of many test results, so these specimens usually need to be rejected; but some specimens are in specific Under the environment, it can be tested after remarks.
The first and most common type is blood samples from newborns. Newborns have thin blood vessels and sometimes low birth weight, which makes it more difficult to collect blood. Hemolysis is difficult to avoid even if blood is collected again, so it is often not rejected.
The second category is patients with intravascular hemolysis, hemolysis specimens caused by heart valve and large blood vessel surgery, such as re-sampling blood may cause new damage or impact to the patient, so hemolysis has little effect on the test items after communicating with the clinician. Not rejected.
The third type, the degree of hemolysis is lighter, and the specimens that have little effect on the results are not rejected.
On the other hand, although hemolysis and lipemia will affect the results of the experiment, the degree of impact is different. Hemolysis is due to the rupture of red blood cells and the release of hemoglobin inside, which affects proteins and other items; because the potassium ion level in the cell is much higher than that of the cell In addition, hemolysis will also affect the detection of electrolyte items.
Therefore, biochemical testing is a task of great responsibility. It is necessary to start from the actual situation and apply the theory to life. Desheng Biochemical has been engaged in the blood testing reagent industry for many years, and believes that the practitioners of biochemical testing are also worthy of respect and admiration.
When it comes to the chemiluminescent reagent Luminol, it is not unfamiliar to everyone and is commonly used in the field of criminal investigation to detect blood stains. Researchers have found that the principle of luminol luminescence can be used to detect specific substances in saliva, such as proteins, DNA, and related indicators of microorganisms, in order to determine physical conditions.