Requirements for buffer system in complement protein research

In vitro studies have shown that the stability and functional activity of complement proteins are greatly influenced by environmental pH. Small fluctuations in pH may lead to changes in protein conformation, decreased enzyme activity, or non-specific precipitation, thereby affecting the accuracy of experimental results. Therefore, choosing a buffer that is pH stable and biocompatible under physiological conditions is crucial for complement related experiments. MOPSO buffer(CAS number: 68399-77-9) has gradually become a commonly used choice in complement protein research due to its suitable buffer range and good chemical stability.

MOPSO powder

Physical and chemical properties and applicability of MOPSO

The pKa value of MOPSO is approximately 7.2 (25 ° C), with an effective buffering range of pH 6.5-7.9, which is close to physiological pH and suitable for maintaining the activity of most complement proteins. At 37 ° C, the pH changes little with temperature, which helps to maintain system stability during constant temperature experiments.

MOPSO belongs to the category of zwitterionic buffering agents, and its molecules are generally electrically neutral, making it difficult for them to significantly interact with metal ions (such as Ca ² ⁺, Mg ² ⁺) in solution. And these metal ions are essential cofactors for the classical and alternative pathways of complement. Using a buffer system that does not interfere with the ion state helps maintain the normal progression of complement cascade reactions.

In addition, MOPSO has good water solubility and is not easy to crystallize, making it suitable for preparing buffer solutions for long-term storage. Its chemical structure is stable and not easily decomposed under common experimental conditions, making it suitable for various biochemical analysis scenarios.

The practical application of MOPSO in complement experiments

In hemolysis tests, MOPSO can be used to prepare reaction buffer to maintain pH stability during the co incubation of red blood cells and complement proteins, reduce non-specific hemolysis, and improve detection reproducibility.

During the purification process of complement proteins, MOPSO buffer can be used for chromatographic elution and preservation, which helps maintain the solubility and functional activity of the target protein and reduce aggregation.

In immune detection methods such as ELISA or Western blot, MOPSO can be used as a component of dilution or washing buffer to reduce background interference and improve the clarity of detection signals.

In complement related drug screening, a stable buffer environment helps to accurately evaluate the inhibitory or regulatory effects of candidate compounds, supporting in vitro pharmacological studies.

Product packaging

Hubei Xindesheng: Manufacturer of MOPSO buffer

Desheng New Materials focuses on the research and production of biological buffering agents, and can provide other biological buffering agent products such as MOPSO (CAS: 68399-77-9). The company has standardized production processes and quality control systems, and its product purity meets industry application requirements. It is widely used in scientific research, diagnostic reagent development, and other fields. Hubei Xindesheng supports bulk supply and basic technical services, committed to providing stable and reliable buffer raw materials for life science research. If you need to know product details or obtain technical information, you can directly contact for support.