In the field of modern agricultural science, with the development of biotechnology and genetics, more and more research is dedicated to improving crop stress resistance, yield, and nutritional value. Among them, seabuckthorn, as an important ecological restoration plant and a high nutritional value fruit source, its genetic engineering research is particularly important. This article will explore the key role of 2-methylmorpholine ethanesulfonic acid (MES buffer) in seabuckthorn genetic engineering and demonstrate how it can become a powerful assistant in seabuckthorn genetic breeding research.

MES buffer

Chemical properties and application prospects of MES

MES is a substance that appears as a white solid powder at room temperature and pressure, and its molecular structure contains a morpholine ring and an ethanesulfonic acid group. This combination endows MES with excellent chemical stability and good water solubility, making it an ideal buffer and promoter. Of particular note is that the ethanesulfonic acid group of MES carries a negative charge, which not only increases its water solubility but also enhances its ability as a buffer, ensuring stable pH values in complex biochemical reaction environments. It is precisely these characteristics that make MES an indispensable and important component in agricultural biotechnology.

The breakthrough path of seabuckthorn genetic engineering

Sea buckthorn is a woody oil tree species with characteristics such as rapid growth, drought tolerance, and salt alkali tolerance, widely used in soil and water conservation and desert control projects. In addition, the fruits, leaves, and stems of seabuckthorn are rich in various bioactive compounds that are beneficial to the human body and have high nutritional and medicinal value. However, for a long time, there have been relatively few reports on the genetic transformation of seabuckthorn, which has greatly limited the research on seabuckthorn genetic engineering. In order to overcome this bottleneck, researchers have begun exploring new methods of genetic modification.

Application of MES in Agrobacterium mediated genetic transformation of seabuckthorn

A patent proposes a new method for Agrobacterium mediated genetic transformation of seabuckthorn by soaking flowers. This method utilizes Agrobacterium tumefaciens LBA4404 containing the mCherry reporter gene to immerse pollinated seabuckthorn female inflorescences in Agrobacterium tumefaciens solution containing MES, achieving highly intuitive and stable genetic transformation. In this process, MES is not only one of the key components in preparing Agrobacterium tumefaciens soaking solution, but also plays an important role in maintaining the pH stability of the solution. In addition, the presence of MES also promotes the interaction between Agrobacterium and seabuckthorn cells, improves gene transfer efficiency, and provides strong support for the functional gene validation of seabuckthorn.

MES helps seabuckthorn genetic engineering research move towards a new stage

The use of MES as a component of Agrobacterium tumefaciens soaking solution not only significantly shortened the transformation cycle of seabuckthorn, but also achieved real-time monitoring of gene expression through fluorescent labeling, laying a solid foundation for subsequent research. The application of MES marks a new level in the genetic engineering research of seabuckthorn, which not only helps to solve the current challenges, but also opens up new paths for cultivating better seabuckthorn varieties in the future.

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Hubei Xindesheng: A professional supplier of MES

When looking for a reliable supplier of 2-morpholine ethanesulfonic acid (MES), Hubei Xindesheng is undoubtedly a trustworthy choice. Whether for scientific research or large-scale industrial applications, choosing Desheng's MES products is a firm commitment to quality and performance. Through continuous technological innovation and service optimization, Hubei Xindesheng not only helps customers succeed, but also contributes to the progress of the entire industry. Join us, explore more possibilities together, and embrace a better tomorrow together.