Accurate measurement of serum total cholesterol (TC) levels is crucial for risk assessment of cardiovascular disease in modern medicine and health management. HDAOS reagent, as a chromogenic substrate, plays an important role in cholesterol enzymatic determination. This article will provide a detailed introduction to the entire process from sample collection to detection result generation, and explore the advantages of HDAOS in detecting cholesterol.

HDAOS powder

Collection and processing of samples

Proper collection and processing of samples is an important step in ensuring the accuracy of experimental results before conducting any laboratory tests. For the detection of serum total cholesterol, it is common to use vacuum blood collection tubes and anticoagulant tubes to collect venous blood. To prevent blood clotting, blood collection tubes containing dipotassium EDTA or heparin as anticoagulants are usually selected. The collected samples need to be gently inverted and mixed 5 to 8 times to ensure even distribution of anticoagulants and avoid result bias caused by blood cell sedimentation.

HDAOS detects cholesterol in samples

Total cholesterol includes two parts: free cholesterol and cholesterol esters. During the detection process, cholesterol esters in the serum are hydrolyzed into free cholesterol and free fatty acids through the action of cholesterol esterase (CHE). Next, under the action of cholesterol oxidase (CHO), free cholesterol is oxidized to produce 4-cholesterenone and hydrogen peroxide. Then, under the catalysis of peroxidase, HDAOS and 4-aminoantipyrine (4-AAP) are coupled for oxidation to form red quinone imine compounds. The color intensity generated by this series of reactions directly reflects the level of TC content in the sample, which can be quantitatively analyzed by measuring its absorbance with a spectrophotometer.

The advantages of using HDAOS to detect cholesterol

There are many methods for determining serum total cholesterol, including chemical reagent colorimetric method, enzyme analysis method, fluorescence method, and gas phase and high performance liquid chromatography method. However, enzymatic determination of total cholesterol using chromogenic substrates such as HDAOS is currently considered a common method. This method has several significant advantages, including:

Fast and accurate: The HDAOS detection method can complete the determination of a large number of samples in a short period of time, while providing high-precision results. Due to its specific enzymatic reaction, this method has strong specificity and can effectively reduce interference from non target substances.

Simplified operational process: Compared to other complex technical methods such as gas phase and high-performance liquid chromatography, HDAOS detection does not require tedious sample pretreatment steps or extraction of lipid components, simplifying the experimental process and reducing the possibility of human error

Adapting to automated platforms: With the improvement of laboratory automation, the HDAOS detection method is very suitable for integration into automated analytical instruments due to its simplicity and stability, thus achieving large-scale screening.

Cost effectiveness: Due to the small sample size and lack of specific equipment support, the HDAOS detection method also performs well economically, making large-scale population health monitoring possible.

Product packaging

In summary, HDAOS detection of serum total cholesterol is an application that integrates advanced scientific and technological achievements. Hubei Xindesheng specializes in the research and production of HDAOS and other various chromogenic substrates, which not only improves the speed and accuracy of detection, but also provides more favorable prices as a direct manufacturer. What are you waiting for? Hurry up and place an order with me.