In today's increasingly sophisticated technology of biological separation and analysis, the selection of buffer solution is crucial. MES (2- (N-morpholino) ethanesulfonic acid), as a widely used zwitterionic buffer, plays an important role in various chromatographic and electrophoretic systems due to its excellent buffering performance, good water solubility, and wide applicability, especially in acidic pH environments.

MES powder

What is MES? Overview of Basic Characteristics

The chemical name of MES is 2- (N-morpholino) ethanesulfonic acid, with a molecular weight of 195.2 g/mol, an effective pH buffer range of 5.5 to 6.7, and a pKa value of 6.10 at 25 ° C. This pH range precisely covers many key areas in biochemical reactions and protein separation processes, making it an ideal buffer choice under low to moderate acidic conditions. MES also has the following advantages:

1. Good water solubility: easy to quickly prepare buffer solutions of specific concentrations;

2. Low UV absorption rate: suitable for high performance liquid chromatography (HPLC) or capillary electrophoresis that rely on UV detection;

3. Low chelating ability for metal ions: reduces the impact on enzyme activity or metal related proteins;

4. Strong thermal stability: can adapt to experimental needs under different temperature conditions.

Diverse application scenarios: from capillary electrochromatography to cation exchange chromatography

Ideal buffer components in capillary electrochromatography (CEC)

In capillary electrochromatography, MES is often used as a part of the background electrolyte to adjust the system pH and maintain conductivity stability. Due to its excellent buffering ability within the acidic range and its ability to not interfere with the migration behavior of the sample, it is particularly suitable for separating positively charged peptides or small molecule compounds.

2. Common buffer systems in gel filtration chromatography

Gel filtration chromatography (GFC) is a separation technology based on molecular size. MES buffer can effectively maintain a constant pH of the solution during this process, preventing target proteins from denaturing or aggregating due to environmental changes, thereby ensuring consistency and repeatability of separation efficiency.

3. Optimal buffer for phosphocellulose column chromatography

Phosphocellulose column chromatography is commonly used for the purification of nucleotides, RNA, or certain specific proteins. MES can provide a stable ionic environment at appropriate pH, which facilitates the binding and elution of target substances on the medium, while avoiding non-specific adsorption.

4. Auxiliary buffer in hydrophobic interaction chromatography (HIC)

In hydrophobic interaction chromatography, MES can be used to adjust the ion strength and pH of the mobile phase, promote the selective binding and release of target proteins on hydrophobic media, and thus improve separation efficiency.

5. Standard configuration in cation exchange chromatography

MES is one of the commonly used buffer solutions for cation exchange chromatography systems that require operation in acidic environments. It can not only maintain a stable pH environment, but also effectively regulate the elution gradient, improving the recovery rate and purity of the target protein.

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Desheng New Materials: Professional production, quality assurance

As a professional supplier of diagnostic reagent raw materials and biological buffering agents in China, Desheng New Materials has been deeply involved in the industry for many years, focusing on the research and production of high-quality MES and other Good's series buffering agents. We have a comprehensive quality control system and advanced production processes to ensure that every batch of products has high purity, batch consistency, and high solubility. What are you waiting for? Contact us to purchase now!