Maintaining the stability of the pH value of the culture medium is one of the important factors in ensuring the healthy development of plants during tissue culture and seedling growth. How to maintain a constant pH environment and reduce the pressure on plants caused by changes in acidity or alkalinity. MES buffer (2-morpholinoethanesulfonic acid) plays a crucial role as an effective pH stabilizer in plant cultivation, like a silently dedicated "invisible guardian" providing guarantees for the healthy growth of plant cells.

MES buffer

Stable pH value, building a solid foundation for growth

Plant cells are extremely sensitive to the pH value in the culture medium, and the ideal pH range (approximately 5.5 to 6.7) is crucial for plants to absorb nutrients, carry out photosynthesis, and respiration. However, as plants grow, their roots release substances such as polysaccharides, amino acids, and organic acids into the culture medium, which can cause changes in the pH value of the medium, usually a decrease. The experimental results showed that without the use of buffering agents, the pH value of the culture medium may decrease by 1.5 units, which directly interferes with the normal growth of plants.

MES buffer, with its excellent buffering performance, can quickly adjust the hydrogen ion concentration and fix the pH value of the culture medium at about 5.8, effectively combating pH fluctuations caused by root exudates. This stability not only prevents metabolic abnormalities caused by pH changes, but also creates a stable growth condition for plants, allowing cells to focus on energy conversion and substance synthesis.

Optimize metabolism and stimulate growth potential

In addition to stabilizing the pH value, MES buffer can also assist plant growth by regulating the activity of internal metabolic enzymes in plants. Research has found that the addition of MES can reduce the activity of phenylalanine ammonia lyase (PAL) and peroxidase (POD) in plants. PAL is involved in the metabolism of phenolic compounds, and its activity is closely related to plant disease resistance; POD, as a key enzyme in the reactive oxygen species defense system, although helps to eliminate superoxide radicals and hydrogen peroxide, excessive activity may lead to chlorophyll decomposition and cell aging. The role of MES leads to a decrease in PAL and POD activity, allowing plants to concentrate more energy on growth rather than defense. This' load reduction 'effect enables plant seedlings to utilize resources more efficiently and achieve rapid growth.

Enhance resilience and help survive in adversity

Plants inevitably encounter various harsh conditions during their life cycle, such as drought, salinization, pests and diseases. MES buffer indirectly enhances plant stress tolerance by maintaining the stability of the culture medium. For example, a stable pH environment is beneficial for plants to absorb mineral elements such as calcium and magnesium more quickly. These elements are crucial for plant cell walls and membrane structures, helping to enhance the mechanical strength of cells. In addition, MES buffer may regulate hormone balance in plants, promote the expression of stress tolerance related genes, and make plants more resilient in the face of adverse environments.

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Desheng quality empowers plant cultivation

Desheng New Materials provides stable and reliable support. As a professional manufacturer in the field of biological buffering agents, Desheng New Materials understands the core value of MES buffering agents in plant cultivation. We use advanced production technology and strict quality monitoring system to ensure that every batch of MES buffer meets the standards of high purity and low impurities. Whether applied in biochemical testing, in vitro diagnostics, cell culture or plant cultivation, the MES buffer of Desheng New Materials can provide you with solid and reliable support.