TAE or TBE？ Guidelines for selecting nucleic acid electrophoresis buffer solutions
Release time:
2025-05-19
In the research field of biochemistry and molecular biology, gel electrophoresis technology is an indispensable part. Through this method, scientists are able to separate DNA fragments of different sizes and shapes for subsequent analysis. The buffer solutions used, mainly TAE (Tris acetic acid) and TBE (Tris boric acid), are crucial for the success of the experiment. Below, we will delve into the characteristics, advantages, disadvantages, and applications of these two buffer solutions.
TAE: Widely used and highly adaptable
Let's first learn about TAE. The migration rate of supercoiled DNA in TAE corresponds well with the molecular weight of linear DNA, making it particularly suitable for plasmid conformation analysis. Research has shown that the electrophoresis speed of double stranded linear DNA in TAE is about 10% faster than other buffer solutions, which is particularly valuable for time sensitive experiments. When the DNA fragment exceeds 13kb, TAE can provide clearer band separation. This is because the low ionic strength of acetate ion reduces the electrostatic interaction between nucleic acid and gel, and reduces the "retention effect" of large-scale DNA.
Due to the absence of interfering substances such as borates, the DNA fragments obtained from TAE electrophoresis can be directly used in gel recovery kits, with generally higher recovery rates than TBE. This is also why the TAE system is recommended for experiments such as enzyme digestion detection and PCR product purification.
TBE: High resolution but challenging
Next, let's take a look at TBE. TBE is known for its large buffer capacity, which makes it particularly outstanding when processing small fragments of DNA smaller than 1kb, providing higher resolution. However, TBE also faces some challenges. Due to its low solubility, TBE is not easy to store for a long time and is prone to precipitation. More importantly, borate ions in TBE may interfere with subsequent enzymatic reactions and reduce the recovery rate of DNA fragments. Therefore, in experiments that require rubber cutting and recycling, it is generally not recommended to use TBE.
Nevertheless, TBE remains an ideal choice for daily testing experiments, such as detecting the quality of DNA, plasmids, or RNA extracted from samples. This is because TBE can provide higher resolution, which helps to clearly distinguish various components in the sample.
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Conclusion
In summary, both TAE and TBE have their own advantages and are suitable for different experimental needs. However, in the pursuit of efficient and accurate scientific research, we are always looking for methods that can further optimize the experimental process. Desheng New Materials focuses on the research and production of biological buffering agents. Its TAE and TBE buffer products are far superior to other manufacturers in terms of purity, stability, and effectiveness. Whether pursuing efficient separation of large nucleic acid molecules or emphasizing high-resolution general detection experiments, Desheng New Materials can provide you with more and better buffer solution options, making your experimental journey smoother and more unobstructed.
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