The advantages of chemiluminescence in the detection of new coronavirus antibodies
The new coronavirus that appeared at the end of 2019 can be said to be frightening to hear. This virus disease is referred to as COVID-19. It is a new infectious disease that spreads rapidly and threatens global health; the clinical manifestations of the disease range from mild to severe. Acute respiratory distress syndrome varies. In addition, there are a considerable number of asymptomatic infections, which increases the uncertainty of diagnosis. Laboratory testing plays a key role in the diagnosis and treatment of COVID-19. The current gold standard for COVID-19 diagnosis is to detect viral nucleic acids in patients’ respiratory specimens through real-time reverse transcription polymerase chain reaction. However, the diagnostic accuracy of rRT-PCR is affected by many factors before and during analysis. The detection of specific COVID-19 antibodies can be used as a supplementary, non-invasive method in disease detection and treatment.
Antibody detection is a powerful supplement to the detection of nucleic acid of the new coronavirus. The antibody detection kits currently on the market mainly adopt chemiluminescence and colloidal gold methods. The difference in the clinical use of these two methods is an important issue that many clinicians care about.
Recently, the Laboratory Department of the Third People's Hospital of Kunming City used a chemiluminescence kit to detect a series of clinical samples at different stages of infection, and evaluated the clinical application value of the chemiluminescence method for detecting new coronavirus antibodies.
The new coronavirus antibody determination kit (chemiluminescence method) is based on the principle of double antigen sandwich method to detect the total antibody of new coronavirus. The chemiluminescent microparticle immunoassay (CMIA) is used to qualitatively detect the new coronavirus antibodies in human serum or plasma on the Caris system. First, mix the sample, the magnetic particles coated with the new coronavirus recombinant antigen, and the reaction diluent. The new coronavirus antibody in the sample will bind to the magnetic particles coated with the new coronavirus recombinant antigen. After washing, add the acridinium ester labeled with the new coronavirus recombinant antigen to form a complex of "new coronavirus recombinant antigen-coated magnetic particles-2019-nCoV antibody-2019-nCoV antigen labeled acridinium ester". After washing again to remove substances not bound to the magnetic particles, add pre-excitation liquid and excitation liquid. The resulting chemiluminescence reaction signal is then measured and expressed in relative luminescence units. The amount of new coronavirus antibodies present in the sample is positively correlated with the relative luminescence units detected on the Caris system.
Researchers found through research and comparison that chemiluminescence kits can recognize all types of 2019-nCoV specific antibodies, including IgM, IgG, IgA, etc., while colloidal gold kits can only recognize 2019-nCoV specific IgM or IgG antibodies. In addition, the chemiluminescence method judges the result by collecting photons and reading the luminescence value, while the colloidal gold method is only judged by the naked eye and is easily affected by subjective factors. The chemiluminescence method is more sensitive than the colloidal gold method.
The chemiluminescence method used in the detection of new coronavirus antibodies has high sensitivity and high degree of albino in the detection system, which helps to reduce the detection workload of medical workers and reduces the advantages of detection risks, and can meet the needs of clinical diagnosis of new coronavirus infection cases. Desheng currently produces inactivated and non-inactivated virus transporattion medium that can meet a large amount of viral nucleic acid for testing, and can also provide antigen-labeled acridinium esters as a powerful supplement for nucleic acid detection. Desheng will definitely move forward in the fight against the new crown .
HEPES, as a zwitterionic buffer, increases the osmotic pressure of the cell culture system by increasing the concentration of solution ions, maintaining normal cell morphology and function, and improving cell survival rate. Widely used in cell culture, especially under specific conditions such as tumor cell culture, it is crucial to maintain cell growth and function.